6ni3: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-'''Unreleased structure'''
-The entry 6ni3 is ON HOLD
+==B2V2R-Gs protein subcomplex of a GPCR-G protein-beta-arrestin mega-complex==
+<SX load='6ni3' size='340' side='right' viewer='molstar' caption='[[6ni3]], [[Resolution|resolution]] 3.80&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6ni3]] is a 5 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterobacteria_phage_RB59 Enterobacteria phage RB59], [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Lama_glama Lama glama]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6NI3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6NI3 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 3.8&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=P0G:8-[(1R)-2-{[1,1-DIMETHYL-2-(2-METHYLPHENYL)ETHYL]AMINO}-1-HYDROXYETHYL]-5-HYDROXY-2H-1,4-BENZOXAZIN-3(4H)-ONE'>P0G</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ni3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ni3 OCA], [https://pdbe.org/6ni3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ni3 RCSB], [https://www.ebi.ac.uk/pdbsum/6ni3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ni3 ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Classically, G-protein-coupled receptors (GPCRs) are thought to activate G protein from the plasma membrane and are subsequently desensitized by beta-arrestin (beta-arr). However, some GPCRs continue to signal through G protein from internalized compartments, mediated by a GPCR-G protein-beta-arr 'megaplex'. Nevertheless, the molecular architecture of the megaplex remains unknown. Here, we present its cryo-electron microscopy structure, which shows simultaneous engagement of human G protein and bovine beta-arr to the core and phosphorylated tail, respectively, of a single active human chimeric beta2-adrenergic receptor with the C-terminal tail of the arginine vasopressin type 2 receptor (beta2V2R). All three components adopt their canonical active conformations, suggesting that a single megaplex GPCR is capable of simultaneously activating G protein and beta-arr. Our findings provide a structural basis for GPCR-mediated sustained internalized G protein signaling.
-Authors:
+Structure of an endosomal signaling GPCR-G protein-beta-arrestin megacomplex.,Nguyen AH, Thomsen ARB, Cahill TJ 3rd, Huang R, Huang LY, Marcink T, Clarke OB, Heissel S, Masoudi A, Ben-Hail D, Samaan F, Dandey VP, Tan YZ, Hong C, Mahoney JP, Triest S, Little J 4th, Chen X, Sunahara R, Steyaert J, Molina H, Yu Z, des Georges A, Lefkowitz RJ Nat Struct Mol Biol. 2019 Nov 18. pii: 10.1038/s41594-019-0330-y. doi:, 10.1038/s41594-019-0330-y. PMID:31740855<ref>PMID:31740855</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6ni3" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Transducin 3D structures|Transducin 3D structures]]
+== References ==
+<references/>
+__TOC__
+</SX>
+[[Category: Enterobacteria phage RB59]]
+[[Category: Homo sapiens]]
+[[Category: Lama glama]]
+[[Category: Large Structures]]
+[[Category: Cahill TJ]]
+[[Category: Lefkowitz RJ]]
+[[Category: Nguyen AH]]
+[[Category: Thomsen ARB]]
+[[Category: Des Georges A]]