6hhe: Difference between revisions
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==Crystal structure of the medfly Odorant Binding Protein CcapOBP22/CcapOBP69a== | ==Crystal structure of the medfly Odorant Binding Protein CcapOBP22/CcapOBP69a== | ||
<StructureSection load='6hhe' size='340' side='right' caption='[[6hhe]], [[Resolution|resolution]] 1.52Å' scene=''> | <StructureSection load='6hhe' size='340' side='right'caption='[[6hhe]], [[Resolution|resolution]] 1.52Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6hhe]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HHE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HHE FirstGlance]. <br> | <table><tr><td colspan='2'>[[6hhe]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cerca Cerca]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HHE OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HHE FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">OBP69a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7213 CERCA])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hhe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hhe OCA], [http://pdbe.org/6hhe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hhe RCSB], [http://www.ebi.ac.uk/pdbsum/6hhe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hhe ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hhe FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hhe OCA], [http://pdbe.org/6hhe PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hhe RCSB], [http://www.ebi.ac.uk/pdbsum/6hhe PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hhe ProSAT]</span></td></tr> | ||
</table> | </table> | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Cerca]] | |||
[[Category: Large Structures]] | |||
[[Category: Ciossani, G]] | [[Category: Ciossani, G]] | ||
[[Category: Falchetto, M]] | [[Category: Falchetto, M]] | ||
Latest revision as of 02:03, 6 June 2019
Crystal structure of the medfly Odorant Binding Protein CcapOBP22/CcapOBP69aCrystal structure of the medfly Odorant Binding Protein CcapOBP22/CcapOBP69a
Structural highlights
Publication Abstract from PubMedIn insects, odorant-binding proteins (OBPs) connect the peripheral sensory system to receptors of olfactory organs. Medfly Ceratitis capitata CcapObp22 shows 37% identity and close phylogenetic affinities with Drosophila melanogaster OBP69a/PB-PRP1 (pheromone-binding protein related protein 1). The CcapObp22 gene is transcribed in the antennae and maxillary palps, suggesting an active role in olfaction. Here, we recombinantly produced CcapObp22, obtaining a 13.5 kDa protein capable of binding multiple strongly hydrophobic terpene compounds, including medfly male pheromone components. The highest binding affinity (EC50 0.48 muM) was to (E,E)-alpha-farnesene, one of the most abundant compounds in the male pheromone blend. This odorant was used in co-crystallization experiments, yielding the structure of CcapOBP22. The monomeric structure shows the typical OBP folding, constituted by six alpha-helical elements interconnected by three disulfide bridges. A C-terminal seventh alpha-helix constitutes the wall of a deep, L-shaped hydrophobic cavity. Analysis of the electron density in this cavity suggested trapping of farnesene in the crystal structure, although with partial occupancy. Superposition of the CcapOBP22 structure with related 7-helical OBPs highlights striking similarity in the organization of the C-terminal segment of these proteins. Collectively, our molecular and physiological data on medfly CcapOBP22 suggest its involvement in inter-sex olfactory communication. This article is protected by copyright. All rights reserved. Structural and biochemical evaluation of Ceratitis capitata OBP22 affinity for odorants involved in inter-sex communication.,Falchetto M, Ciossani G, Scolari F, Di Cosimo A, Nenci S, Field LM, Mattevi A, Zhou JJ, Gasperi G, Forneris F Insect Mol Biol. 2018 Dec 12. doi: 10.1111/imb.12559. PMID:30548711[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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