6bbf: Difference between revisions
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==The CRAC channel Orai in an open conformation; H206A gain-of-function mutation== | ==The CRAC channel Orai in an open conformation; H206A gain-of-function mutation== | ||
<StructureSection load='6bbf' size='340' side='right' caption='[[6bbf]], [[Resolution|resolution]] 6.71Å' scene=''> | <StructureSection load='6bbf' size='340' side='right'caption='[[6bbf]], [[Resolution|resolution]] 6.71Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6bbf]] is a 24 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BBF OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6bbf]] is a 24 chain structure with sequence from [https://en.wikipedia.org/wiki/Drosophila_melanogaster Drosophila melanogaster]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BBF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BBF FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 6.706Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6bbf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6bbf OCA], [https://pdbe.org/6bbf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6bbf RCSB], [https://www.ebi.ac.uk/pdbsum/6bbf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6bbf ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/CRCM1_DROME CRCM1_DROME] Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores. Regulates transcription factor NFAT nuclear import.<ref>PMID:16582901</ref> <ref>PMID:16645049</ref> | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Drosophila melanogaster]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Burstein S]] | ||
[[Category: | [[Category: Hou X]] | ||
[[Category: | [[Category: Long SB]] | ||
Latest revision as of 13:08, 16 August 2023
The CRAC channel Orai in an open conformation; H206A gain-of-function mutationThe CRAC channel Orai in an open conformation; H206A gain-of-function mutation
Structural highlights
FunctionCRCM1_DROME Ca(2+) release-activated Ca(2+) (CRAC) channel subunit which mediates Ca(2+) influx following depletion of intracellular Ca(2+) stores. Regulates transcription factor NFAT nuclear import.[1] [2] Publication Abstract from PubMedThe store-operated calcium (Ca(2+)) channel Orai governs Ca(2+) influx through the plasma membrane of many non-excitable cells in metazoans. The channel opens in response to the depletion of Ca(2+) stored in the endoplasmic reticulum (ER). Loss- and gain-of-function mutants of Orai cause disease. Our previous work revealed the structure of Orai with a closed pore. Here, using a gain-of-function mutation that constitutively activates the channel, we present an X-ray structure of Drosophila melanogaster Orai in an open conformation. Well-defined electron density maps reveal that the pore is dramatically dilated on its cytosolic side in comparison to the slender closed pore. Cations and anions bind in different regions of the open pore, informing mechanisms for ion permeation and Ca(2+) selectivity. Opening of the pore requires the release of cytosolic latches. Together with additional X-ray structures of an unlatched-but-closed conformation, we propose a sequence for store-operated activation. Structures reveal opening of the store-operated calcium channel Orai.,Hou X, Burstein SR, Long SB Elife. 2018 Aug 30;7. pii: 36758. doi: 10.7554/eLife.36758. PMID:30160233[3] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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