6hk5: Difference between revisions
New page: '''Unreleased structure''' The entry 6hk5 is ON HOLD Authors: Alfano, M., Perard, J., Basset, C., Carpentier, P., Zambelli, B., Timm, J., Crouzy, S., Ciurli, S., Cavazza, C. Descriptio... |
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==X-ray structure of a truncated mutant of the metallochaperone CooJ with a high-affinity nickel-binding site== | |||
<StructureSection load='6hk5' size='340' side='right'caption='[[6hk5]], [[Resolution|resolution]] 2.04Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6hk5]] is a 8 chain structure with sequence from [http://en.wikipedia.org/wiki/"dicrospirillum_rubrum"_enderlein_1925 "dicrospirillum rubrum" enderlein 1925]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HK5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HK5 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene>, <scene name='pdbligand=Z3P:3,3,3-phosphoryltripropanoic+acid'>Z3P</scene></td></tr> | |||
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cooJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1085 "Dicrospirillum rubrum" Enderlein 1925])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hk5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hk5 OCA], [http://pdbe.org/6hk5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hk5 RCSB], [http://www.ebi.ac.uk/pdbsum/6hk5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hk5 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Activation of nickel enzymes requires specific accessory proteins organized in multiprotein complexes controlling metal transfer to the active site. Histidine-rich clusters are generally present in at least one of the metallochaperones involved in nickel delivery. The maturation of carbon monoxide dehydrogenase in the proteobacterium Rhodospirillum rubrum requires three accessory proteins, CooC, CooT, and CooJ, dedicated to nickel insertion into the active site, a distorted [NiFe3S4] cluster coordinated to an iron site. Previously, CooJ from R. rubrum (RrCooJ) has been described as a nickel chaperone with 16 histidines and 2 cysteines at its C terminus. Here, the X-ray structure of a truncated version of RrCooJ, combined with small-angle X-ray scattering data and a modeling study of the full-length protein, revealed a homodimer comprising a coiled coil with two independent and highly flexible His tails. Using isothermal calorimetry, we characterized several metal-binding sites (four per dimer) involving the His-rich motifs and having similar metal affinity (KD = 1.6 mum). Remarkably, biophysical approaches, site-directed mutagenesis, and X-ray crystallography uncovered an additional nickel-binding site at the dimer interface, which binds Ni(II) with an affinity of 380 nm Although RrCooJ was initially thought to be a unique protein, a proteome database search identified at least 46 bacterial CooJ homologs. These homologs all possess two spatially separated nickel-binding motifs: a variable C-terminal histidine tail and a strictly conserved H(W/F)X 2HX 3H motif, identified in this study, suggesting a dual function for CooJ both as a nickel chaperone and as a nickel storage protein. | |||
The carbon monoxide dehydrogenase accessory protein CooJ is a histidine-rich multidomain dimer containing an unexpected Ni(II)-binding site.,Alfano M, Perard J, Carpentier P, Basset C, Zambelli B, Timm J, Crouzy S, Ciurli S, Cavazza C J Biol Chem. 2019 May 10;294(19):7601-7614. doi: 10.1074/jbc.RA119.008011. Epub, 2019 Mar 11. PMID:30858174<ref>PMID:30858174</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6hk5" style="background-color:#fffaf0;"></div> | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Dicrospirillum rubrum enderlein 1925]] | |||
[[Category: Large Structures]] | |||
[[Category: Alfano, M]] | |||
[[Category: Basset, C]] | |||
[[Category: Carpentier, P]] | |||
[[Category: Cavazza, C]] | [[Category: Cavazza, C]] | ||
[[Category: Ciurli, S]] | |||
[[Category: Crouzy, S]] | [[Category: Crouzy, S]] | ||
[[Category: Perard, J]] | [[Category: Perard, J]] | ||
[[Category: Timm, J]] | [[Category: Timm, J]] | ||
[[Category: | [[Category: Zambelli, B]] | ||
[[Category: | [[Category: Metal binding protein]] | ||
[[Category: | [[Category: Metallochaperone]] | ||
[[Category: Nickel-binding]] | |||