6cqs: Difference between revisions
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==Sediminispirochaeta smaragdinae SPS-1 metallo-beta-lactamase== | ==Sediminispirochaeta smaragdinae SPS-1 metallo-beta-lactamase== | ||
<StructureSection load='6cqs' size='340' side='right' caption='[[6cqs]], [[Resolution|resolution]] 1.70Å' scene=''> | <StructureSection load='6cqs' size='340' side='right'caption='[[6cqs]], [[Resolution|resolution]] 1.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[6cqs]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CQS OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[6cqs]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Sediminispirochaeta_smaragdinae_DSM_11293 Sediminispirochaeta smaragdinae DSM 11293]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CQS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6CQS FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.7Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6cqs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6cqs OCA], [https://pdbe.org/6cqs PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6cqs RCSB], [https://www.ebi.ac.uk/pdbsum/6cqs PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6cqs ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/E1R245_SEDSS E1R245_SEDSS] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 6cqs" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 6cqs" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Beta-lactamase 3D structures|Beta-lactamase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Sediminispirochaeta smaragdinae DSM 11293]] | ||
[[Category: | [[Category: Cheng Z]] | ||
[[Category: | [[Category: Crowder MW]] | ||
[[Category: | [[Category: Page RC]] | ||
[[Category: | [[Category: VanPelt J]] | ||
Latest revision as of 18:09, 4 October 2023
Sediminispirochaeta smaragdinae SPS-1 metallo-beta-lactamaseSediminispirochaeta smaragdinae SPS-1 metallo-beta-lactamase
Structural highlights
FunctionPublication Abstract from PubMedIn an effort to evaluate whether a recently reported putative metallo-beta-lactamase (MbetaL) contains a novel MbetaL active site, SPS-1 from Sediminispirochaeta smaragdinae was overexpressed, purified, and characterized using spectroscopic and crystallographic studies. Metal analyses demonstrate that recombinant SPS-1 binds nearly 2 equiv of Zn(II), and steady-state kinetic studies show that the enzyme hydrolyzes carbapenems and certain cephalosporins but not beta-lactam substrates with bulky substituents at the 6/7 position. Spectroscopic studies of Co(II)-substituted SPS-1 suggest a novel metal center in SPS-1, with a reduced level of spin coupling between the metal ions and a novel Zn1 metal binding site. This site was confirmed with a crystal structure of the enzyme. The structure shows a Zn2 site that is similar to that in NDM-1 and other subclass B1 MbetaLs; however, the Zn1 metal ion is coordinated by two histidine residues and a water molecule, which is held in position by a hydrogen bond network. The Zn1 metal is displaced nearly 1 A from the position reported in other MbetaLs. The structure also shows extended helices above the active site, which create a binding pocket that precludes the binding of substrates with large, bulky substituents at the 6/7 position of beta-lactam antibiotics. This study reveals a novel metal binding site in MbetaLs and suggests that the targeting of metal binding sites in MbetaLs with inhibitors is now more challenging with the identification of this new MbetaL. A Noncanonical Metal Center Drives the Activity of the Sediminispirochaeta smaragdinae Metallo-beta-lactamase SPS-1.,Cheng Z, VanPelt J, Bergstrom A, Bethel C, Katko A, Miller C, Mason K, Cumming E, Zhang H, Kimble RL, Fullington S, Bretz SL, Nix JC, Bonomo RA, Tierney DL, Page RC, Crowder MW Biochemistry. 2018 Aug 21. doi: 10.1021/acs.biochem.8b00728. PMID:30106565[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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