6hd3: Difference between revisions
New page: '''Unreleased structure''' The entry 6hd3 is ON HOLD until Paper Publication Authors: Heinemann, U., Roske, Y., Banchenko, S., Arumughan, A., Petrovic, S. Description: Common mode of r... |
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==Common mode of remodeling AAA ATPases p97/CDC48 by their disassembly cofactors ASPL/PUX1== | |||
<StructureSection load='6hd3' size='340' side='right'caption='[[6hd3]], [[Resolution|resolution]] 2.80Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6hd3]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Arath Arath]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HD3 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HD3 FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ADP:ADENOSINE-5-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr> | |||
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">CDC48A, CDC48, At3g09840, F8A24.11 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3702 ARATH])</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hd3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hd3 OCA], [http://pdbe.org/6hd3 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hd3 RCSB], [http://www.ebi.ac.uk/pdbsum/6hd3 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hd3 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[[http://www.uniprot.org/uniprot/CD48A_ARATH CD48A_ARATH]] Probably functions in cell division and growth processes. Interacts with certain SNAREs as part of specialized membrane fusion events where vesicles from the same organelle fuse (homotypic fusion) (By similarity). | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
The hexameric ring structure of the type II AAA+ ATPases is considered as stable and permanent. Recently, the UBX domain-containing cofactors Arabidopsis thaliana PUX1 and human alveolar soft part sarcoma locus (ASPL) were reported to bind and disassemble the cognate AAA+ ATPases AtCDC48 and human p97. Here, we present two crystal structures related to these complexes: a truncated AtCDC48 (AtCDC48-ND1) and a hybrid complex containing human p97-ND1 and the UBX domain of plant PUX1 (p97-ND1:PUX1-UBX). These structures reveal close similarity between the human and plant AAA+ ATPases, but also highlight differences between disassembling and non-disassembling AAA+ ATPase cofactors. Based on an AtCDC48 disassembly assay with PUX1 and known crystal structures of the p97-bound human cofactor ASPL, we propose a general ATPase disassembly model. Thus, our structural and biophysical investigations provide detailed insight into the mechanism of AAA+ ATPase disassembly by UBX domain cofactors and suggest a general mode of regulating the cellular activity of these molecular machines. | |||
Common Mode of Remodeling AAA ATPases p97/CDC48 by Their Disassembling Cofactors ASPL/PUX1.,Banchenko S, Arumughan A, Petrovic S, Schwefel D, Wanker EE, Roske Y, Heinemann U Structure. 2019 Oct 21. pii: S0969-2126(19)30342-9. doi:, 10.1016/j.str.2019.10.001. PMID:31648844<ref>PMID:31648844</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6hd3" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Arath]] | |||
[[Category: Large Structures]] | |||
[[Category: Arumughan, A]] | |||
[[Category: Banchenko, S]] | |||
[[Category: Heinemann, U]] | [[Category: Heinemann, U]] | ||
[[Category: Petrovic, S]] | [[Category: Petrovic, S]] | ||
[[Category: Roske, Y]] | [[Category: Roske, Y]] | ||
[[Category: | [[Category: Arabidopsis thaliana]] | ||
[[Category: Atpase]] | |||
[[Category: Cdc48]] | |||
[[Category: Hexamer]] | |||
[[Category: Plant protein]] | |||