6hck: Difference between revisions

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-'''Unreleased structure'''
-The entry 6hck is ON HOLD
+==The Transcriptional Regulator PrfA from Listeria Monocytogenes in complex with dipeptide Leu-Leu==
+<StructureSection load='6hck' size='340' side='right'caption='[[6hck]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6hck]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Lismo Lismo]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6HCK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6HCK FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=LEU:LEUCINE'>LEU</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">prfA, lmo0200 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=169963 LISMO])</td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6hck FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6hck OCA], [http://pdbe.org/6hck PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6hck RCSB], [http://www.ebi.ac.uk/pdbsum/6hck PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6hck ProSAT]</span></td></tr>
+</table>
+== Function ==
+[[http://www.uniprot.org/uniprot/PRFA_LISMO PRFA_LISMO]] Positively regulates expression of listeriolysin, of 1-phosphadidylinositol phosphodiesterase (PI-PLC) and other virulence factors.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+To optimize fitness, pathogens selectively activate their virulence program upon host entry. Here, we report that the facultative intracellular bacterium Listeria monocytogenes exploits exogenous oligopeptides, a ubiquitous organic N source, to sense the environment and control the activity of its virulence transcriptional activator, PrfA. Using a genetic screen in adsorbent-treated (PrfA-inducing) medium, we found that PrfA is functionally regulated by the balance between activating and inhibitory nutritional peptides scavenged via the Opp transport system. Activating peptides provide essential cysteine precursor for the PrfA-inducing cofactor glutathione (GSH). Non-cysteine-containing peptides cause promiscuous PrfA inhibition. Biophysical and co-crystallization studies reveal that peptides inhibit PrfA through steric blockade of the GSH binding site, a regulation mechanism directly linking bacterial virulence and metabolism. L. monocytogenes mutant analysis in macrophages and our functional data support a model in which changes in the balance of antagonistic Opp-imported oligopeptides promote PrfA induction intracellularly and PrfA repression outside the host.
-Authors:
+Control of Bacterial Virulence through the Peptide Signature of the Habitat.,Krypotou E, Scortti M, Grundstrom C, Oelker M, Luisi BF, Sauer-Eriksson AE, Vazquez-Boland J Cell Rep. 2019 Feb 12;26(7):1815-1827.e5. doi: 10.1016/j.celrep.2019.01.073. PMID:30759392<ref>PMID:30759392</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6hck" style="background-color:#fffaf0;"></div>
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Lismo]]
+[[Category: Grundstrom, C]]
+[[Category: Krypotou, E]]
+[[Category: Luisi, B F]]
+[[Category: Oelker, M]]
+[[Category: Sauer-Eriksson, A E]]
+[[Category: Scortti, M]]
+[[Category: Vazquez-Boland, J]]
+[[Category: Dipeptide]]
+[[Category: Dna binding protein]]
+[[Category: Listeria inhibition]]
+[[Category: Prfa]]
+[[Category: Virulence]]