Phosphocarrier protein: Difference between revisions

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<StructureSection load='' size='350' side='right' caption='Structure of phosphocarrier protein HPr (PDB entry [[1fu0]])' scene='57/570591/Cv/1'>
<StructureSection load='' size='350' side='right' caption='Structure of phosphocarrier protein HPr (PDB entry [[1fu0]])' scene='57/570591/Cv/1'>
__TOC__
== Function ==   
== Function ==   
'''Phosphocarrier protein HPr''' (HPr) is a component of the sugar phosphotransferase system.  The system catalyses the phosphorylation of sugars during their translocation across cell membrane.  A phosphoryl group is transferred from phosphoenolpyruvate via several enzymes (EI, EII) to HPr<ref>PMID:8246840</ref>.  A conserved histidine in the N terminal of HPr is the acceptor of the phosphate.  In Gram-positive bacteria HPr there is a conserved serine which is phosphorylated.
'''Phosphocarrier protein HPr''' (HPr) is a component of the sugar phosphotransferase system.  The system catalyses the phosphorylation of sugars during their translocation across cell membrane.  A phosphoryl group is transferred from phosphoenolpyruvate via several enzymes (EI, EII) to HPr<ref>PMID:8246840</ref>.  A conserved histidine in the N terminal of HPr is the acceptor of the phosphate.  In Gram-positive bacteria HPr there is a conserved serine which is phosphorylated.
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== Structural highlights ==
== Structural highlights ==
The <scene name='57/570591/Cv/2'>phosphorylation of Ser46</scene> prohibits the HPr to act as a phosphocarrier in the EI reaction<ref>PMID:11054290</ref>.
The <scene name='57/570591/Cv/3'>phosphorylation of Ser46</scene> prohibits the HPr to act as a phosphocarrier in the EI reaction<ref>PMID:11054290</ref>.
</StructureSection>


==3D structures of phosphocarrier protein HPr==
==3D structures of phosphocarrier protein HPr==
[[Phosphocarrier protein HPr 3D structures]]


Updated on {{REVISIONDAY2}}-{{MONTHNAME|{{REVISIONMONTH}}}}-{{REVISIONYEAR}}
</StructureSection>
{{#tree:id=OrganizedByTopic|openlevels=0|
 
*Phosphocarrier protein HPr
 
**[[2hpr]] - BsHPr (mutant) – ''Bacillus subtilis'' <br />
**[[2hid]] - BsHPr (mutant) – NMR <br />
**[[1jem]] - BsHPr (mutant) with phosphohistidine – NMR <br />
**[[1sph]] – BsHPr <br />
**[[1poh]] – EcHPr – ''Escherichia coli''  <br />
**[[1opd]], [[1cm2]], [[1cm3]], [[3ccd]] - EcHPr (mutant)  <br />
**[[1hdn]] - EcHPr – NMR  <br />
**[[1pfh]] - EcHPr with phosphohistidine – NMR  <br />
**[[1ptf]] - EfHPr – ''Enterococcus faecalis'' <br />
**[[1qfr]] - EfHPr – NMR <br />
**[[1fu0]] - EfHPr with phosphoserine  <br />
**[[1pch]] – HPr – ''Mycoplasma capricolum''<br />
**[[1qr5]] - ScHPr – ''Staphylococcus carnosus'' - NMR <br />
**[[1txe]] - ScHPr (mutant) – NMR <br />
**[[1ka5]] - HPr – ''Staphylococcus aureus'' - NMR <br />
**[[1y4y]] – GsHPr – ''Geobacillus stearothermophilus''<br />
**[[1y50]], [[1y51]] - GsHPr (mutant)  <br />
**[[3ihs]] – HPr – ''Bacillus anthracis'' <br />
 
*Phosphocarrier protein HPr complex
 
**[[2jel]] - EcHPr + antibody <br />
**[[3eze]], [[3ezb]], [[3eza]] - EcHPr + EI N terminal + PO3 – NMR  <br />
**[[2xdf]] - EcHPr + EI N terminal – NMR  <br />
**[[1ggr]], [[1j6t]], [[1vrc]] - EcHPr + EII N terminal + PO3 – NMR  <br />
**[[2lrk]], [[2lrl]] - EcHPr + EII N terminal – NMR  <br />
**[[4xwj]] - EcHPr + antisigma factor RSD  <br />
**[[5ya0]] - EcHPr + autoinducer-2 kinase <br />
**[[5ya1]] - EcHPr + autoinducer-2 kinase + ATP<br />
**[[5ya2]] - EcHPr + autoinducer-2 kinase + ADP<br />
**[[1kkl]] - BsHPr (mutant) + HPrK <br />
**[[1kkm]] - BsHPr (mutant) with phosphoserine + HPrK/P <br />
**[[2fep]] - BsHPr with phosphoserine + BsCcpA <br />
**[[1rzr]] – BmHPr with phosphoserine + BmCcpA + DNA  - ''Bacillus megaterium''<br />
**[[2oen]] - BmHPr with phosphoserine + BmCcpA<br />
**[[2nzu]] - BmHPr with phosphoserine + BmCcpA + glucose-6-phosphate  <br />
**[[2nzv]] - BmHPr with phosphoserine + BmCcpA + fructose-1,6-diphosphate  <br />
**[[3oqm]], [[3oqn]], [[3oqo]] - BsHPr with phosphoserine + BsCcpA + DNA<br />
 
*Phosphocarrier protein NPr
 
**[[5t17]], [[5t1n]] - EcNPr – NMR  <br />
**[[5t1o]] - EcNPr + EIN-NTR – NMR  <br />
 
*Phosphocarrier protein III Glc
 
**[[1f3g]] - EcPrcpIII  <br />


}}
== References ==
== References ==
<references/>
<references/>
[[Category:Topic Page]]
[[Category:Topic Page]]

Latest revision as of 11:36, 17 November 2019

Function

Phosphocarrier protein HPr (HPr) is a component of the sugar phosphotransferase system. The system catalyses the phosphorylation of sugars during their translocation across cell membrane. A phosphoryl group is transferred from phosphoenolpyruvate via several enzymes (EI, EII) to HPr[1]. A conserved histidine in the N terminal of HPr is the acceptor of the phosphate. In Gram-positive bacteria HPr there is a conserved serine which is phosphorylated.

Phosphocarrier protein NPr (NPr) is a component of the sugar phosphotransferase system. NPr is a phosphoryl transfer protein, homologous to HPr which functions to regulate nitrogen metabolism[2].

Phosphocarrier protein III Glc (PcpIII) is a signal-transducing component of the phosphoenolpyruvate:glycose phosphotransferase system of E. coli[3].

Structural highlights

The prohibits the HPr to act as a phosphocarrier in the EI reaction[4].

3D structures of phosphocarrier protein HPr

Phosphocarrier protein HPr 3D structures


Structure of phosphocarrier protein HPr (PDB entry 1fu0)

Drag the structure with the mouse to rotate

ReferencesReferences

  1. ↑ Postma PW, Lengeler JW, Jacobson GR. Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria. Microbiol Rev. 1993 Sep;57(3):543-94. PMID:8246840
  2. ↑ Li X, Peterkofsky A, Wang G. Solution structure of NPr, a bacterial signal-transducing protein that controls the phosphorylation state of the potassium transporter-regulating protein IIA Ntr. Amino Acids. 2008 Oct;35(3):531-9. doi: 10.1007/s00726-008-0079-9. Epub 2008 Apr , 18. PMID:18421563 doi:http://dx.doi.org/10.1007/s00726-008-0079-9
  3. ↑ Maurer T, Doker R, Gorler A, Hengstenberg W, Kalbitzer HR. Three-dimensional structure of the histidine-containing phosphocarrier protein (HPr) from Enterococcus faecalis in solution. Eur J Biochem. 2001 Feb;268(3):635-44. PMID:11168402
  4. ↑ Audette GF, Engelmann R, Hengstenberg W, Deutscher J, Hayakawa K, Quail JW, Delbaere LT. The 1.9 A resolution structure of phospho-serine 46 HPr from Enterococcus faecalis. J Mol Biol. 2000 Nov 3;303(4):545-53. PMID:11054290 doi:10.1006/jmbi.2000.4166

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