6d39: Difference between revisions
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New page: '''Unreleased structure''' The entry 6d39 is ON HOLD Authors: Zhou, X., Fan, L., Lin, M. Description: Photodissociable dimeric Dronpa green fluorescent protein variant V (pdDronpaV) [[... |
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==Photodissociable dimeric Dronpa green fluorescent protein variant V (pdDronpaV)== | |||
<StructureSection load='6d39' size='340' side='right'caption='[[6d39]], [[Resolution|resolution]] 2.83Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6d39]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Echinophyllia_sp._SC22 Echinophyllia sp. SC22]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D39 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6D39 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.83Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6d39 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d39 OCA], [https://pdbe.org/6d39 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6d39 RCSB], [https://www.ebi.ac.uk/pdbsum/6d39 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6d39 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q5TLG6_9CNID Q5TLG6_9CNID] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Protein kinases transduce signals to regulate a wide array of cellular functions in eukaryotes. A generalizable method for optical control of kinases would enable fine spatiotemporal interrogation or manipulation of these various functions. We report the design and application of single-chain cofactor-free kinases with photoswitchable activity. We engineered a dimeric protein, pdDronpa, that dissociates in cyan light and reassociates in violet light. Attaching two pdDronpa domains at rationally selected locations in the kinase domain, we created the photoswitchable kinases psRaf1, psMEK1, psMEK2, and psCDK5. Using these photoswitchable kinases, we established an all-optical cell-based assay for screening inhibitors, uncovered a direct and rapid inhibitory feedback loop from ERK to MEK1, and mediated developmental changes and synaptic vesicle transport in vivo using light. | |||
Optical control of cell signaling by single-chain photoswitchable kinases.,Zhou XX, Fan LZ, Li P, Shen K, Lin MZ Science. 2017 Feb 24;355(6327):836-842. doi: 10.1126/science.aah3605. PMID:28232577<ref>PMID:28232577</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 6d39" style="background-color:#fffaf0;"></div> | ||
[[Category: Lin | |||
[[Category: | ==See Also== | ||
*[[Dronpa|Dronpa]] | |||
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Echinophyllia sp. SC22]] | |||
[[Category: Large Structures]] | |||
[[Category: Fan L]] | |||
[[Category: Lin M]] | |||
[[Category: Zhou X]] | |||
Latest revision as of 18:17, 4 October 2023
Photodissociable dimeric Dronpa green fluorescent protein variant V (pdDronpaV)Photodissociable dimeric Dronpa green fluorescent protein variant V (pdDronpaV)
Structural highlights
FunctionPublication Abstract from PubMedProtein kinases transduce signals to regulate a wide array of cellular functions in eukaryotes. A generalizable method for optical control of kinases would enable fine spatiotemporal interrogation or manipulation of these various functions. We report the design and application of single-chain cofactor-free kinases with photoswitchable activity. We engineered a dimeric protein, pdDronpa, that dissociates in cyan light and reassociates in violet light. Attaching two pdDronpa domains at rationally selected locations in the kinase domain, we created the photoswitchable kinases psRaf1, psMEK1, psMEK2, and psCDK5. Using these photoswitchable kinases, we established an all-optical cell-based assay for screening inhibitors, uncovered a direct and rapid inhibitory feedback loop from ERK to MEK1, and mediated developmental changes and synaptic vesicle transport in vivo using light. Optical control of cell signaling by single-chain photoswitchable kinases.,Zhou XX, Fan LZ, Li P, Shen K, Lin MZ Science. 2017 Feb 24;355(6327):836-842. doi: 10.1126/science.aah3605. PMID:28232577[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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