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| [[Image:2gt1.jpg|left|200px]]
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| {{Structure
| | ==E. coli heptosyltransferase WaaC.== |
| |PDB= 2gt1 |SIZE=350|CAPTION= <scene name='initialview01'>2gt1</scene>, resolution 1.900Å
| | <StructureSection load='2gt1' size='340' side='right'caption='[[2gt1]], [[Resolution|resolution]] 1.90Å' scene=''> |
| |SITE=
| | == Structural highlights == |
| |LIGAND=
| | <table><tr><td colspan='2'>[[2gt1]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_UTI89 Escherichia coli UTI89]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GT1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GT1 FirstGlance]. <br> |
| |ACTIVITY=
| | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> |
| |GENE=
| | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gt1 OCA], [https://pdbe.org/2gt1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gt1 RCSB], [https://www.ebi.ac.uk/pdbsum/2gt1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gt1 ProSAT]</span></td></tr> |
| |DOMAIN=
| | </table> |
| |RELATEDENTRY=
| | == Function == |
| |RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gt1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gt1 OCA], [http://www.ebi.ac.uk/pdbsum/2gt1 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gt1 RCSB]</span>
| | [https://www.uniprot.org/uniprot/Q1R4X0_ECOUT Q1R4X0_ECOUT] |
| }}
| | == Evolutionary Conservation == |
| | | [[Image:Consurf_key_small.gif|200px|right]] |
| '''E. coli heptosyltransferase WaaC.'''
| | Check<jmol> |
| | | <jmolCheckbox> |
| | | <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gt/2gt1_consurf.spt"</scriptWhenChecked> |
| ==Overview== | | <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked> |
| Lipopolysaccharides constitute the outer leaflet of the outer membrane of Gram-negative bacteria and are therefore essential for cell growth and viability. The heptosyltransferase WaaC is a glycosyltransferase (GT) involved in the synthesis of the inner core region of LPS. It catalyzes the addition of the first L-glycero-D-manno-heptose (heptose) molecule to one 3-deoxy-D-manno-oct-2-ulosonic acid (Kdo) residue of the Kdo2-lipid A molecule. Heptose is an essential component of the LPS core domain; its absence results in a truncated lipopolysaccharide associated with the deep-rough phenotype causing a greater susceptibility to antibiotic and an attenuated virulence for pathogenic Gram-negative bacteria. Thus, WaaC represents a promising target in antibacterial drug design. Here, we report the structure of WaaC from the Escherichia coli pathogenic strain RS218 alone at 1.9 A resolution, and in complex with either ADP or the non-cleavable analog ADP-2-deoxy-2-fluoro-heptose of the sugar donor at 2.4 A resolution. WaaC adopts the GT-B fold in two domains, characteristic of one glycosyltransferase structural superfamily. The comparison of the three different structures shows that WaaC does not undergo a domain rotation, characteristic of the GT-B family, upon substrate binding, but allows the substrate analog and the reaction product to adopt remarkably distinct conformations inside the active site. In addition, both binary complexes offer a close view of the donor subsite and, together with results from site-directed mutagenesis studies, provide evidence for a model of the catalytic mechanism.
| | <text>to colour the structure by Evolutionary Conservation</text> |
| | | </jmolCheckbox> |
| ==About this Structure== | | </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gt1 ConSurf]. |
| 2GT1 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli_uti89 Escherichia coli uti89]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GT1 OCA].
| | <div style="clear:both"></div> |
| | | __TOC__ |
| ==Reference== | | </StructureSection> |
| Structure of the Escherichia coli heptosyltransferase WaaC: binary complexes with ADP and ADP-2-deoxy-2-fluoro heptose., Grizot S, Salem M, Vongsouthi V, Durand L, Moreau F, Dohi H, Vincent S, Escaich S, Ducruix A, J Mol Biol. 2006 Oct 20;363(2):383-94. Epub 2006 Jul 29. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16963083 16963083]
| | [[Category: Escherichia coli UTI89]] |
| [[Category: Escherichia coli uti89]] | | [[Category: Large Structures]] |
| [[Category: Single protein]] | | [[Category: Dohi H]] |
| [[Category: Dohi, H.]] | | [[Category: Ducruix A]] |
| [[Category: Ducruix, A.]] | | [[Category: Durand L]] |
| [[Category: Durand, L.]] | | [[Category: Escaich S]] |
| [[Category: Escaich, S.]] | | [[Category: Grizot S]] |
| [[Category: Grizot, S.]] | | [[Category: Moreau F]] |
| [[Category: Moreau, F.]] | | [[Category: Salem M]] |
| [[Category: Salem, M.]] | | [[Category: Vincent S]] |
| [[Category: Vincent, S.]] | | [[Category: Vongsouthi V]] |
| [[Category: Vongsouthi, V.]] | |
| [[Category: gt-b fold]]
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| ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:21:09 2008''
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