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[[Image:2gh7.gif|left|200px]]


{{Structure
==Epi-biotin complex with core streptavidin==
|PDB= 2gh7 |SIZE=350|CAPTION= <scene name='initialview01'>2gh7</scene>, resolution 1.00&Aring;
<StructureSection load='2gh7' size='340' side='right'caption='[[2gh7]], [[Resolution|resolution]] 1.00&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=BTQ:EPI-BIOTIN'>BTQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
<table><tr><td colspan='2'>[[2gh7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GH7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2GH7 FirstGlance]. <br>
|ACTIVITY=  
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1&#8491;</td></tr>
|GENE=  
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=BTQ:EPI-BIOTIN'>BTQ</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
|DOMAIN=
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2gh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gh7 OCA], [https://pdbe.org/2gh7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2gh7 RCSB], [https://www.ebi.ac.uk/pdbsum/2gh7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2gh7 ProSAT]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gh7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gh7 OCA], [http://www.ebi.ac.uk/pdbsum/2gh7 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gh7 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/gh/2gh7_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2gh7 ConSurf].
<div style="clear:both"></div>


'''Epi-biotin complex with core streptavidin'''
==See Also==
 
*[[Avidin 3D structures|Avidin 3D structures]]
 
__TOC__
==Overview==
</StructureSection>
(+)-Epi-biotin differs from (+)-biotin in the configuration of the chiral center at atom C2. This could lead to a difference in the mode of binding of (+)-epi-biotin to streptavidin, a natural protein receptor for (+)-biotin. Diffraction data were collected to a maximum of 0.85 Angstrom resolution for structural analysis of the complex of streptavidin with a sample of (+)-epi-biotin and refinement was carried out at both 1.0 and 0.85 Angstrom resolution. The structure determination shows a superposition of two ligands in the binding site, (+)-biotin and (+)-epi-biotin. The molecules overlap in the model for the complex except for the position of S1 in the tetrahydrothiophene ring. Differences in the conformation of the ring permits binding of each molecule to streptavidin with little observable difference in the protein structures at this high resolution.
[[Category: Large Structures]]
 
==About this Structure==
2GH7 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GH7 OCA].
 
==Reference==
The high-resolution structure of (+)-epi-biotin bound to streptavidin., Le Trong I, Aubert DG, Thomas NR, Stenkamp RE, Acta Crystallogr D Biol Crystallogr. 2006 Jun;62(Pt 6):576-81. Epub 2006, May 12. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16699183 16699183]
[[Category: Single protein]]
[[Category: Streptomyces avidinii]]
[[Category: Streptomyces avidinii]]
[[Category: Aubert, D G.L.]]
[[Category: Aubert DGL]]
[[Category: Stenkamp, R E.]]
[[Category: Le Trong I]]
[[Category: Thomas, N R.]]
[[Category: Stenkamp RE]]
[[Category: Trong, I Le.]]
[[Category: Thomas NR]]
[[Category: biotin]]
[[Category: protein/ligand interaction]]
[[Category: streptavidin]]
 
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:16:36 2008''

Latest revision as of 12:27, 14 February 2024

Epi-biotin complex with core streptavidinEpi-biotin complex with core streptavidin

Structural highlights

2gh7 is a 2 chain structure with sequence from Streptomyces avidinii. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1Å
Ligands:, ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

SAV_STRAV The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2gh7, resolution 1.00Å

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