1e3o: Difference between revisions
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==Crystal structure of Oct-1 POU dimer bound to MORE== | ==Crystal structure of Oct-1 POU dimer bound to MORE== | ||
<StructureSection load='1e3o' size='340' side='right' caption='[[1e3o]], [[Resolution|resolution]] 1.90Å' scene=''> | <StructureSection load='1e3o' size='340' side='right'caption='[[1e3o]], [[Resolution|resolution]] 1.90Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1e3o]] is a 3 chain structure with sequence from [ | <table><tr><td colspan='2'>[[1e3o]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1E3O OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1E3O FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.9Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1e3o FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1e3o OCA], [https://pdbe.org/1e3o PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1e3o RCSB], [https://www.ebi.ac.uk/pdbsum/1e3o PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1e3o ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/PO2F1_HUMAN PO2F1_HUMAN] Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.<ref>PMID:1684878</ref> | ||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: Pohl | [[Category: Large Structures]] | ||
[[Category: Remenyi | [[Category: Pohl E]] | ||
[[Category: Schoeler | [[Category: Remenyi A]] | ||
[[Category: Tomilin | [[Category: Schoeler H]] | ||
[[Category: Wilmanns | [[Category: Tomilin A]] | ||
[[Category: Wilmanns M]] | |||
Latest revision as of 14:51, 13 December 2023
Crystal structure of Oct-1 POU dimer bound to MORECrystal structure of Oct-1 POU dimer bound to MORE
Structural highlights
FunctionPO2F1_HUMAN Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity). In case of human herpes simplex virus (HSV) infection, POU2F1 forms a multiprotein-DNA complex with the viral transactivator protein VP16 and HCFC1 thereby enabling the transcription of the viral immediate early genes.[1] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedTwo crystal structures of Oct-1 POU domain bound to DNA provide a rationale for differential, conformation-dependent recruitment of transcription cofactors. The POU-homeo and POU-specific subdomains of Oct-1 contain two different nonoverlapping pairs of surface patches that are capable of forming unrelated protein-protein interfaces. Members of the POU factor family contain one or two conserved sequence motifs in the interface that are known to be phosphorylated, as noted for Oct-1 and Pit-1. Modeling of Oct-4 reveals the unique case where the same conserved sequence is located in both interfaces. Our studies provide the basis for two distinct dimeric POU factor arrangements that are dictated by the architecture of each DNA response element. We suggest interface swapping in dimers could be a general mechanism of modulating the activity of transcription factors. Differential dimer activities of the transcription factor Oct-1 by DNA-induced interface swapping.,Remenyi A, Tomilin A, Pohl E, Lins K, Philippsen A, Reinbold R, Scholer HR, Wilmanns M Mol Cell. 2001 Sep;8(3):569-80. PMID:11583619[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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