5ze9: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-'''Unreleased structure'''
-The entry 5ze9 is ON HOLD
+==Crystal structure of AMP-PNP bound mutant A3B3 complex from Enterococcus hirae V-ATPase==
+<StructureSection load='5ze9' size='340' side='right'caption='[[5ze9]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5ze9]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Enterococcus_hirae_ATCC_9790 Enterococcus hirae ATCC 9790]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5ZE9 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5ZE9 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.102&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ze9 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ze9 OCA], [https://pdbe.org/5ze9 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ze9 RCSB], [https://www.ebi.ac.uk/pdbsum/5ze9 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ze9 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/NTPA_ENTHA NTPA_ENTHA] Involved in ATP-driven sodium extrusion.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+V1-ATPase is an ATP-driven rotary motor that is composed of a ring-shaped A3B3 complex and a central DF shaft. The nucleotide-free A3B3 complex of Enterococcus hirae, composed of three identical A1B1 heterodimers, showed a unique asymmetrical structure, probably due to the strong binding of the N-terminal barrel domain, which forms a crown structure. Here, we mutated the barrel region to weaken the crown, and performed structural analyses using high-speed atomic force microscopy and x-ray crystallography of the mutant A3B3. The nucleotide-free mutant A3B3 complex had a more symmetrical open structure than the wild type. Binding of nucleotides produced a closely packed spiral-like structure with a disrupted crown. These findings suggest that wild-type A3B3 forms a metastable (stressed) asymmetric structure composed of unstable A1B1 conformers due to the strong constraint of the crown. The results further the understanding of the principle of the cooperative transition mechanism of rotary motors.
-Authors: Maruyama, S., Suzuki, K., Sasaki, H., Mizutani, K., Saito, Y., Imai, F.L., Ishizuka-Katsura, Y., Shirouzu, M., Ichiro, Y., Murata, T.
+Metastable asymmetrical structure of a shaftless V1 motor.,Maruyama S, Suzuki K, Imamura M, Sasaki H, Matsunami H, Mizutani K, Saito Y, Imai FL, Ishizuka-Katsura Y, Kimura-Someya T, Shirouzu M, Uchihashi T, Ando T, Yamato I, Murata T Sci Adv. 2019 Jan 30;5(1):eaau8149. doi: 10.1126/sciadv.aau8149. eCollection 2019, Jan. PMID:30729160<ref>PMID:30729160</ref>
-Description: Crystal structure of AMP-PNP bound mutant A3B3 complex from Enterococcus hirae V-ATPase
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Mizutani, K]]
+<div class="pdbe-citations 5ze9" style="background-color:#fffaf0;"></div>
-[[Category: Maruyama, S]]
-[[Category: Murata, T]]
+==See Also==
-[[Category: Ichiro, Y]]
+*[[ATPase 3D structures|ATPase 3D structures]]
-[[Category: Imai, F.L]]
+== References ==
-[[Category: Ishizuka-Katsura, Y]]
+<references/>
-[[Category: Suzuki, K]]
+__TOC__
-[[Category: Saito, Y]]
+</StructureSection>
-[[Category: Shirouzu, M]]
+[[Category: Enterococcus hirae ATCC 9790]]
-[[Category: Sasaki, H]]
+[[Category: Large Structures]]
+[[Category: Ichiro Y]]
+[[Category: Imai FL]]
+[[Category: Ishizuka-Katsura Y]]
+[[Category: Maruyama S]]
+[[Category: Mizutani K]]
+[[Category: Murata T]]
+[[Category: Saito Y]]
+[[Category: Sasaki H]]
+[[Category: Shirouzu M]]
+[[Category: Suzuki K]]