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==Crystal structure of collagen adhesin (CNA) from S. aureus==
==Crystal structure of collagen adhesin (CNA) from S. aureus==
<StructureSection load='2f68' size='340' side='right' caption='[[2f68]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
<StructureSection load='2f68' size='340' side='right'caption='[[2f68]], [[Resolution|resolution]] 1.95&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2f68]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"micrococcus_aureus"_(rosenbach_1884)_zopf_1885 "micrococcus aureus" (rosenbach 1884) zopf 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F68 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2F68 FirstGlance]. <br>
<table><tr><td colspan='2'>[[2f68]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2F68 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2F68 FirstGlance]. <br>
</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">cna ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1280 "Micrococcus aureus" (Rosenbach 1884) Zopf 1885])</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.95&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2f68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f68 OCA], [http://pdbe.org/2f68 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2f68 RCSB], [http://www.ebi.ac.uk/pdbsum/2f68 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2f68 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2f68 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2f68 OCA], [https://pdbe.org/2f68 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2f68 RCSB], [https://www.ebi.ac.uk/pdbsum/2f68 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2f68 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/CNA_STAAU CNA_STAAU]] Mediates attachment of staphylococcal cells to collagen-containing substrata.  
[https://www.uniprot.org/uniprot/CNA_STAAU CNA_STAAU] Mediates attachment of staphylococcal cells to collagen-containing substrata.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f68 ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2f68 ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The structural basis for the association of eukaryotic and prokaryotic protein receptors and their triple-helical collagen ligand remains poorly understood. Here, we present the crystal structures of a high affinity subsegment of the Staphylococcus aureus collagen-binding CNA as an apo-protein and in complex with a synthetic collagen-like triple helical peptide. The apo-protein structure is composed of two subdomains (N1 and N2), each adopting a variant IgG-fold, and a long linker that connects N1 and N2. The structure is stabilized by hydrophobic inter-domain interactions and by the N2 C-terminal extension that complements a beta-sheet on N1. In the ligand complex, the collagen-like peptide penetrates through a spherical hole formed by the two subdomains and the N1-N2 linker. Based on these two structures we propose a dynamic, multistep binding model, called the 'Collagen Hug' that is uniquely designed to allow multidomain collagen binding proteins to bind their extended rope-like ligand.


A 'Collagen Hug' model for Staphylococcus aureus CNA binding to collagen.,Zong Y, Xu Y, Liang X, Keene DR, Hook A, Gurusiddappa S, Hook M, Narayana SV EMBO J. 2005 Dec 21;24(24):4224-36. Epub 2005 Dec 15. PMID:16362049<ref>PMID:16362049</ref>
==See Also==
 
*[[Adhesin 3D structures|Adhesin 3D structures]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 2f68" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Narayana, S V.L]]
[[Category: Large Structures]]
[[Category: Zong, Y]]
[[Category: Staphylococcus aureus]]
[[Category: Beta barrel]]
[[Category: Narayana SVL]]
[[Category: Cell adhesion]]
[[Category: Zong Y]]
[[Category: Domain swap]]

Latest revision as of 12:21, 14 February 2024

Crystal structure of collagen adhesin (CNA) from S. aureusCrystal structure of collagen adhesin (CNA) from S. aureus

Structural highlights

2f68 is a 1 chain structure with sequence from Staphylococcus aureus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.95Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

CNA_STAAU Mediates attachment of staphylococcal cells to collagen-containing substrata.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2f68, resolution 1.95Å

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