2ejf: Difference between revisions

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-[[Image:2ejf.jpg|left|200px]]
- {{Structure
+==Crystal Structure Of The Biotin Protein Ligase (Mutations R48A and K111A) and Biotin Carboxyl Carrier Protein Complex From Pyrococcus Horikoshii OT3==
-|PDB= 2ejf |SIZE=350|CAPTION= <scene name='initialview01'>2ejf</scene>, resolution 2.00&Aring;
+<StructureSection load='2ejf' size='340' side='right'caption='[[2ejf]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-|SITE= <scene name='pdbsite=AC1:Btn+Binding+Site+For+Residue+C+1400'>AC1</scene>, <scene name='pdbsite=AC2:Btn+Binding+Site+For+Residue+A+1401'>AC2</scene>, <scene name='pdbsite=AC3:Btn+Binding+Site+For+Residue+B+1402'>AC3</scene>, <scene name='pdbsite=AC4:Adn+Binding+Site+For+Residue+A+2001'>AC4</scene>, <scene name='pdbsite=AC5:Adn+Binding+Site+For+Residue+B+2002'>AC5</scene> and <scene name='pdbsite=AC6:Gol+Binding+Site+For+Residue+B+3001'>AC6</scene>
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>
+<table><tr><td colspan='2'>[[2ejf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_horikoshii_OT3 Pyrococcus horikoshii OT3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2EJF FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-|GENE= bpl ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii]), bccp ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53953 Pyrococcus horikoshii])
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ADN:ADENOSINE'>ADN</scene>, <scene name='pdbligand=BTN:BIOTIN'>BTN</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2ejf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ejf OCA], [https://pdbe.org/2ejf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2ejf RCSB], [https://www.ebi.ac.uk/pdbsum/2ejf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2ejf ProSAT], [https://www.topsan.org/Proteins/RSGI/2ejf TOPSAN]</span></td></tr>
-|RELATEDENTRY=[[2ejg|2EJG]]
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ejf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ejf OCA], [http://www.ebi.ac.uk/pdbsum/2ejf PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ejf RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/O57883_PYRHO O57883_PYRHO]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/ej/2ejf_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2ejf ConSurf].
+<div style="clear:both"></div>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Biotin protein ligase (BPL) catalyzes the biotinylation of the biotin carboxyl carrier protein (BCCP) only at a special lysine residue. Here we report the first structure of BPL.BCCP complex crystals, which are prepared using two BPL mutants: R48A and R48A/K111A. From a detailed structural characterization, it is likely that the mutants retain functionality as enzymes but have a reduced activity to produce the reaction intermediate biotinyl-5'-AMP. The observed biotin and partly disordered ATP in the mutant structures may act as a non-reactive analog of the substrates or biotinyl-5'-AMP, thereby providing the complex crystals. The four crystallographically independent BPL.BCCP complexes obtained can be classified structurally into three groups: the formation stages 1 and 2 with apo-BCCP and the product stage with biotinylated holo-BCCP. Residues responsible for the complex formation as well as for the biotinylation reaction have been identified. The C-terminal domain of BPL shows especially large conformational changes to accommodate BCCP, suggesting its functional importance. The formation stage 1 complex shows the closest distance between the carboxyl carbon of biotin and the special lysine of BCCP, suggesting its relevance to the unobserved reaction stage. Interestingly, bound ATP and biotin are also seen in the product stage, indicating that the substrates may be recruited into the product stage complex before the release of holo-BCCP, probably for the next reaction cycle. The existence of formation and product stages before and after the reaction stage would be favorable to ensure both the reaction efficiency and the extreme substrate specificity of the biotinylation reaction.
-'''Crystal Structure Of The Biotin Protein Ligase (Mutations R48A and K111A) and Biotin Carboxyl Carrier Protein Complex From Pyrococcus Horikoshii OT3'''
+Protein biotinylation visualized by a complex structure of biotin protein ligase with a substrate.,Bagautdinov B, Matsuura Y, Bagautdinova S, Kunishima N J Biol Chem. 2008 May 23;283(21):14739-50. Epub 2008 Mar 26. PMID:18372281<ref>PMID:18372281</ref>
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
+</div>
+<div class="pdbe-citations 2ejf" style="background-color:#fffaf0;"></div>
-==About this Structure==
+==See Also==
-EJF is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Pyrococcus_horikoshii Pyrococcus horikoshii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EJF OCA].
+*[[Biotin Protein Ligase 3D structures|Biotin Protein Ligase 3D structures]]
-[[Category: Protein complex]]
+== References ==
-[[Category: Pyrococcus horikoshii]]
+<references/>
-[[Category: Bagautdinov, B.]]
+__TOC__
-[[Category: Bagautdinova, S.]]
+</StructureSection>
-[[Category: Kunishima, N.]]
+[[Category: Large Structures]]
-[[Category: Matsuura, Y.]]
+[[Category: Pyrococcus horikoshii OT3]]
-[[Category: RSGI, RIKEN Structural Genomics/Proteomics Initiative.]]
+[[Category: Bagautdinov B]]
-[[Category: biotinylation]]
+[[Category: Bagautdinova S]]
-[[Category: dimer]]
+[[Category: Kunishima N]]
-[[Category: ligase]]
+[[Category: Matsuura Y]]
-[[Category: national project on protein structural and functional analyse]]
-[[Category: nppsfa]]
-[[Category: riken structural genomics/proteomics initiative]]
-[[Category: rsgi]]
-[[Category: structural genomic]]
-[[Category: x-ray diffraction]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 02:50:00 2008''