1of4: Difference between revisions
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==Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate-binding module, TmCBM27== | ==Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate-binding module, TmCBM27== | ||
<StructureSection load='1of4' size='340' side='right' caption='[[1of4]], [[Resolution|resolution]] 1.60Å' scene=''> | <StructureSection load='1of4' size='340' side='right'caption='[[1of4]], [[Resolution|resolution]] 1.60Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[1of4]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[1of4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermotoga_maritima_MSB8 Thermotoga maritima MSB8]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1OF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1OF4 FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1of4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1of4 OCA], [https://pdbe.org/1of4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1of4 RCSB], [https://www.ebi.ac.uk/pdbsum/1of4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1of4 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9RIK9_THEMT Q9RIK9_THEMT] | |||
== Evolutionary Conservation == | == Evolutionary Conservation == | ||
[[Image:Consurf_key_small.gif|200px|right]] | [[Image:Consurf_key_small.gif|200px|right]] | ||
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</div> | </div> | ||
<div class="pdbe-citations 1of4" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 1of4" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[Mannosidase 3D structures|Mannosidase 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Thermotoga maritima MSB8]] | ||
[[Category: Boraston | [[Category: Boraston AB]] | ||
[[Category: | [[Category: Boraston CM]] | ||
[[Category: | [[Category: Davies GJ]] | ||
[[Category: | [[Category: Nurizzo D]] | ||
[[Category: | [[Category: Revett TJ]] | ||
Latest revision as of 11:59, 9 May 2024
Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate-binding module, TmCBM27Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate-binding module, TmCBM27
Structural highlights
FunctionEvolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedThe C-terminal 176 amino acids of a Thermotoga maritima mannanase (Man5) constitute a carbohydrate binding module (CBM) that has been classified into CBM family 27. The isolated CBM27 domain, named TmCBM27, binds tightly (K(a)s 10(5)-10(6) M(-1)) to beta-1, 4-mannooligosaccharides, carob galactomannan, and konjac glucomannan, but not to cellulose (insoluble and soluble) or soluble birchwood xylan. The X-ray crystal structures of native TmCBM27, a TmCBM27-mannohexaose complex, and a TmCBM27-6(3),6(4)-alpha-D-galactosyl-mannopentaose complex at 2.0 A, 1.6 A, and 1.35 A, respectively, reveal the basis of TmCBM27's specificity for mannans. In particular, the latter complex, which is the first structure of a CBM in complex with a branched plant cell wall polysaccharide, illustrates how the architecture of the binding site can influence the recognition of naturally substituted polysaccharides. Structural and thermodynamic dissection of specific mannan recognition by a carbohydrate binding module, TmCBM27.,Boraston AB, Revett TJ, Boraston CM, Nurizzo D, Davies GJ Structure. 2003 Jun;11(6):665-75. PMID:12791255[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences |
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