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==HhaI DNA methyltransferase R163N mutant complex with 13mer GCGC-GMGC oligonucleotide and SAH==
==HhaI DNA methyltransferase R163N mutant complex with 13mer GCGC-GMGC oligonucleotide and SAH==
<StructureSection load='2uyc' size='340' side='right' caption='[[2uyc]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
<StructureSection load='2uyc' size='340' side='right'caption='[[2uyc]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[2uyc]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_x"_pritchett_and_stillman_1919 "bacillus x" pritchett and stillman 1919]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UYC OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2UYC FirstGlance]. <br>
<table><tr><td colspan='2'>[[2uyc]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Haemophilus_haemolyticus Haemophilus haemolyticus] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2UYC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=2UYC FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=5CM:5-METHYL-2-DEOXY-CYTIDINE-5-MONOPHOSPHATE'>5CM</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=SAH:S-ADENOSYL-L-HOMOCYSTEINE'>SAH</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[10mh|10mh]], [[1fjx|1fjx]], [[1hmy|1hmy]], [[1m0e|1m0e]], [[1mht|1mht]], [[1skm|1skm]], [[1svu|1svu]], [[2c7o|2c7o]], [[2c7p|2c7p]], [[2c7q|2c7q]], [[2c7r|2c7r]], [[2hmy|2hmy]], [[3mht|3mht]], [[4mht|4mht]], [[5mht|5mht]], [[6mht|6mht]], [[7mht|7mht]], [[8mht|8mht]], [[9mht|9mht]]</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=2uyc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uyc OCA], [https://pdbe.org/2uyc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=2uyc RCSB], [https://www.ebi.ac.uk/pdbsum/2uyc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=2uyc ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA_(cytosine-5-)-methyltransferase DNA (cytosine-5-)-methyltransferase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.1.1.37 2.1.1.37] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2uyc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2uyc OCA], [http://pdbe.org/2uyc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=2uyc RCSB], [http://www.ebi.ac.uk/pdbsum/2uyc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=2uyc ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/MTH1_HAEPH MTH1_HAEPH]] This methylase recognizes the double-stranded sequence GCGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HhaI endonuclease.  
[https://www.uniprot.org/uniprot/MTH1_HAEPH MTH1_HAEPH] This methylase recognizes the double-stranded sequence GCGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HhaI endonuclease.
== Evolutionary Conservation ==
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
[[Image:Consurf_key_small.gif|200px|right]]
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</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2uyc ConSurf].
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=2uyc ConSurf].
<div style="clear:both"></div>
<div style="clear:both"></div>
==See Also==
*[[DNA methyltransferase 3D structures|DNA methyltransferase 3D structures]]
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Bacillus x pritchett and stillman 1919]]
[[Category: Haemophilus haemolyticus]]
[[Category: Daujotyte, D]]
[[Category: Large Structures]]
[[Category: Grazulis, S]]
[[Category: Synthetic construct]]
[[Category: Base flipping]]
[[Category: Daujotyte D]]
[[Category: Dna methyltransferase]]
[[Category: Grazulis S]]
[[Category: Methyltransferase]]
[[Category: Restriction system]]
[[Category: S-adenosyl-l-methionine]]
[[Category: Transferase]]

Latest revision as of 17:57, 13 December 2023

HhaI DNA methyltransferase R163N mutant complex with 13mer GCGC-GMGC oligonucleotide and SAHHhaI DNA methyltransferase R163N mutant complex with 13mer GCGC-GMGC oligonucleotide and SAH

Structural highlights

2uyc is a 3 chain structure with sequence from Haemophilus haemolyticus and Synthetic construct. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2Å
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

MTH1_HAEPH This methylase recognizes the double-stranded sequence GCGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HhaI endonuclease.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

2uyc, resolution 2.00Å

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