6fij: Difference between revisions
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==Structure of the loading/condensing region (SAT-KS-MAT) of the cercosporin fungal non-reducing polyketide synthase (NR-PKS) CTB1== | |||
<StructureSection load='6fij' size='340' side='right' caption='[[6fij]], [[Resolution|resolution]] 2.77Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[6fij]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Barn_spot_disease_fungus Barn spot disease fungus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6FIJ OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6FIJ FirstGlance]. <br> | |||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTT:2,3-DIHYDROXY-1,4-DITHIOBUTANE'>DTT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr> | |||
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6fik|6fik]]</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6fij FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6fij OCA], [http://pdbe.org/6fij PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6fij RCSB], [http://www.ebi.ac.uk/pdbsum/6fij PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6fij ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Polyketide synthases (PKSs) are microbial multienzymes for the biosynthesis of biologically potent secondary metabolites. Polyketide production is initiated by the loading of a starter unit onto an integral acyl carrier protein (ACP) and its subsequent transfer to the ketosynthase (KS). Initial substrate loading is achieved either by multidomain loading modules or by the integration of designated loading domains, such as starter unit acyltransferases (SAT), whose structural integration into PKS remains unresolved. A crystal structure of the loading/condensing region of the nonreducing PKS CTB1 demonstrates the ordered insertion of a pseudodimeric SAT into the condensing region, which is aided by the SAT-KS linker. Cryo-electron microscopy of the post-loading state trapped by mechanism-based crosslinking of ACP to KS reveals asymmetry across the CTB1 loading/-condensing region, in accord with preferential 1:2 binding stoichiometry. These results are critical for re-engineering the loading step in polyketide biosynthesis and support functional relevance of asymmetric conformations of PKSs. | |||
The structural organization of substrate loading in iterative polyketide synthases.,Herbst DA, Huitt-Roehl CR, Jakob RP, Kravetz JM, Storm PA, Alley JR, Townsend CA, Maier T Nat Chem Biol. 2018 Apr 2. pii: 10.1038/s41589-018-0026-3. doi:, 10.1038/s41589-018-0026-3. PMID:29610486<ref>PMID:29610486</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 6fij" style="background-color:#fffaf0;"></div> | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Barn spot disease fungus]] | |||
[[Category: Herbst, D A]] | |||
[[Category: Jakob, R P]] | |||
[[Category: Maier, T]] | |||
[[Category: Townsend, C A]] | |||
[[Category: Biosynthetic protein]] | |||
[[Category: Condensing]] | |||
[[Category: Fungal]] | |||
[[Category: Ipk]] | |||
[[Category: Iterative pk]] | |||
[[Category: Loading]] | |||
[[Category: Non-reducing]] | |||
[[Category: Nr-pk]] | |||
[[Category: Pk]] | |||
[[Category: Polyketide]] | |||
[[Category: Sat]] | |||
[[Category: Starter acyl]] | |||
Latest revision as of 17:25, 11 April 2018
Structure of the loading/condensing region (SAT-KS-MAT) of the cercosporin fungal non-reducing polyketide synthase (NR-PKS) CTB1Structure of the loading/condensing region (SAT-KS-MAT) of the cercosporin fungal non-reducing polyketide synthase (NR-PKS) CTB1
Structural highlights
Publication Abstract from PubMedPolyketide synthases (PKSs) are microbial multienzymes for the biosynthesis of biologically potent secondary metabolites. Polyketide production is initiated by the loading of a starter unit onto an integral acyl carrier protein (ACP) and its subsequent transfer to the ketosynthase (KS). Initial substrate loading is achieved either by multidomain loading modules or by the integration of designated loading domains, such as starter unit acyltransferases (SAT), whose structural integration into PKS remains unresolved. A crystal structure of the loading/condensing region of the nonreducing PKS CTB1 demonstrates the ordered insertion of a pseudodimeric SAT into the condensing region, which is aided by the SAT-KS linker. Cryo-electron microscopy of the post-loading state trapped by mechanism-based crosslinking of ACP to KS reveals asymmetry across the CTB1 loading/-condensing region, in accord with preferential 1:2 binding stoichiometry. These results are critical for re-engineering the loading step in polyketide biosynthesis and support functional relevance of asymmetric conformations of PKSs. The structural organization of substrate loading in iterative polyketide synthases.,Herbst DA, Huitt-Roehl CR, Jakob RP, Kravetz JM, Storm PA, Alley JR, Townsend CA, Maier T Nat Chem Biol. 2018 Apr 2. pii: 10.1038/s41589-018-0026-3. doi:, 10.1038/s41589-018-0026-3. PMID:29610486[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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