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| ==X-ray crystal structure of AA13 LPMO== | | ==X-ray crystal structure of AA13 LPMO== |
| <StructureSection load='5t7n' size='340' side='right' caption='[[5t7n]], [[Resolution|resolution]] 1.60Å' scene=''> | | <StructureSection load='5t7n' size='340' side='right'caption='[[5t7n]], [[Resolution|resolution]] 1.60Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[5t7n]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aspor Aspor]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T7N OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5T7N FirstGlance]. <br> | | <table><tr><td colspan='2'>[[5t7n]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aspergillus_oryzae_RIB40 Aspergillus oryzae RIB40]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5T7N OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5T7N FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6Å</td></tr> |
| <tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene></td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=HIC:4-METHYL-HISTIDINE'>HIC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AO090701000246 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=510516 ASPOR])</td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5t7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t7n OCA], [https://pdbe.org/5t7n PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5t7n RCSB], [https://www.ebi.ac.uk/pdbsum/5t7n PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5t7n ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5t7n FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5t7n OCA], [http://pdbe.org/5t7n PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5t7n RCSB], [http://www.ebi.ac.uk/pdbsum/5t7n PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5t7n ProSAT]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/AA13_ASPOR AA13_ASPOR] Lytic polysaccharide monooxygenase-related protein that has no activity towards starch.<ref>PMID:25608804</ref> |
| Lytic polysaccharide monooxygenases (LPMOs) are a class of copper-dependent enzymes discovered within the last ten years. They oxidatively cleave polysaccharides (chitin, lignocellulose, hemicellulose and starch-derived), presumably making recalcitrant substrates accessible to glycoside hydrolases. Recently, the first crystal structure of an LPMO-substrate complex was reported, giving insights into the interaction of LPMOs with beta-linked substrates (Frandsen et al., 2016). The LPMOs acting on alpha-linked glycosidic bonds (family AA13) display binding surfaces that are quite different from those of LPMOs that act on beta-linked glycosidic bonds (families AA9-AA11), as revealed from the first determined structure (Lo Leggio et al., 2015), and thus presumably the AA13s interact with their substrate in a distinct fashion. Here, several new structures of the same AA13 enzyme, Aspergillus oryzae AA13, are presented. Crystals obtained in the presence of high zinc-ion concentrations were used, as they can be obtained more reproducibly than those used to refine the deposited copper-containing structure. One structure with an ordered zinc-bound active site was solved at 1.65 A resolution, and three structures from crystals soaked with maltooligosaccharides in solutions devoid of zinc ions were solved at resolutions of up to 1.10 A. Despite similar unit-cell parameters, small rearrangements in the crystal packing occur when the crystals are depleted of zinc ions, resulting in a more occluded substrate-binding surface. In two of the three structures maltooligosaccharide ligands are bound, but not at the active site. Two of the structures presented show a His-ligand conformation that is incompatible with metal-ion binding. In one of these structures this conformation is the principal one (80% occupancy), giving a rare atomic resolution view of a substantially misfolded enzyme that is presumably rendered inactive. | |
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| Learning from oligosaccharide soaks of crystals of an AA13 lytic polysaccharide monooxygenase: crystal packing, ligand binding and active-site disorder.,Frandsen KE, Poulsen JC, Tovborg M, Johansen KS, Lo Leggio L Acta Crystallogr D Struct Biol. 2017 Jan 1;73(Pt 1):64-76. doi:, 10.1107/S2059798316019641. Epub 2017 Jan 1. PMID:28045386<ref>PMID:28045386</ref>
| | ==See Also== |
| | | *[[Monooxygenase 3D structures|Monooxygenase 3D structures]] |
| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 5t7n" style="background-color:#fffaf0;"></div>
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| == References == | | == References == |
| <references/> | | <references/> |
| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Aspor]] | | [[Category: Aspergillus oryzae RIB40]] |
| [[Category: Frandsen, K E.H]] | | [[Category: Large Structures]] |
| [[Category: Johansen, K S]] | | [[Category: Frandsen KEH]] |
| [[Category: Leggio, L Lo]] | | [[Category: Johansen KS]] |
| [[Category: Poulsen, J C.N]] | | [[Category: Lo Leggio L]] |
| [[Category: Tovborg, M]] | | [[Category: Poulsen J-CN]] |
| [[Category: Aspergillus oryzae aa13 lpmo]]
| | [[Category: Tovborg M]] |
| [[Category: Enzyme]]
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| [[Category: Metal binding protein]]
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