6br7: Difference between revisions

Proteopedia Page Contributors and Editors (what is this?)Proteopedia Page Contributors and Editors (what is this?)

OCA

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-'''Unreleased structure'''
-The entry 6br7 is ON HOLD
+==Beryllium fluorinated receiver domain of BfmR from Acinetobacter baumannii==
+<StructureSection load='6br7' size='340' side='right'caption='[[6br7]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6br7]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Acinetobacter_baumannii Acinetobacter baumannii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6BR7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6BR7 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.86&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BEF:BERYLLIUM+TRIFLUORIDE+ION'>BEF</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6br7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6br7 OCA], [https://pdbe.org/6br7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6br7 RCSB], [https://www.ebi.ac.uk/pdbsum/6br7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6br7 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/A0A014C6J9_9GAMM A0A014C6J9_9GAMM]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The rise of drug-resistant bacterial infections coupled with decreasing antibiotic efficacy poses a significant challenge to global healthcare. Acinetobacter baumannii is an insidious, emerging bacterial pathogen responsible for severe nosocomial infections aided by its ability to form biofilms. The response regulator BfmR, from the BfmR/S two-component system, is the master regulator of biofilm initiation in A. baumannii and is a tractable therapeutic target. Here we present the structure of A. baumannii BfmR using a hybrid approach combining X-ray crystallography, nuclear magnetic resonance spectroscopy, chemical crosslinking mass spectrometry, and molecular modeling. We also show that BfmR binds the previously proposed bfmRS promoter sequence with moderate affinity. While BfmR shares many traits with other OmpR/PhoB family response regulators, some unusual properties were observed. Most importantly, we observe that when phosphorylated, BfmR binds this promoter sequence with a lower affinity than when not phosphorylated. All other OmpR/PhoB family members studied to date show an increase in DNA binding affinity upon phosphorylation. Understanding the structural and biochemical mechanisms of BfmR will aid in the development of new antimicrobial therapies.
-Authors: Milton, M.E., Cavanagh, J.
+The structure of the biofilm-controlling response regulator BfmR from Acinetobacter baumannii reveals details of its DNA-binding mechanism.,Logan Draughn G, Milton ME, Feldmann EA, Bobay BG, Roth BM, Olson AL, Thompson RJ, Actis LA, Davies C, Cavanagh J J Mol Biol. 2018 Feb 10. pii: S0022-2836(18)30070-6. doi:, 10.1016/j.jmb.2018.02.002. PMID:29438671<ref>PMID:29438671</ref>
-Description: Beryllium fluorinated receiver domain of BfmR from Acinetobacter baumannii
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Milton, M.E]]
+<div class="pdbe-citations 6br7" style="background-color:#fffaf0;"></div>
-[[Category: Cavanagh, J]]
+==See Also==
+*[[Response regulator 3D structure|Response regulator 3D structure]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Acinetobacter baumannii]]
+[[Category: Large Structures]]
+[[Category: Cavanagh J]]
+[[Category: Milton ME]]