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==Crystal structure of hypothetical protein PF0907 from Pyrococcus furiosus solved by sulfur SAD using Swiss Light Source data== | ==Crystal structure of hypothetical protein PF0907 from Pyrococcus furiosus solved by sulfur SAD using Swiss Light Source data== | ||
<StructureSection load='4pgo' size='340' side='right' caption='[[4pgo]], [[Resolution|resolution]] 2.30Å' scene=''> | <StructureSection load='4pgo' size='340' side='right'caption='[[4pgo]], [[Resolution|resolution]] 2.30Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4pgo]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4pgo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Pyrococcus_furiosus_DSM_3638 Pyrococcus furiosus DSM 3638]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4PGO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4PGO FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4pgo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4pgo OCA], [https://pdbe.org/4pgo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4pgo RCSB], [https://www.ebi.ac.uk/pdbsum/4pgo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4pgo ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8U2D2_PYRFU Q8U2D2_PYRFU] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
| Line 22: | Line 23: | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Chen | [[Category: Pyrococcus furiosus DSM 3638]] | ||
[[Category: Olieric | [[Category: Chen L]] | ||
[[Category: Panepucci | [[Category: Olieric V]] | ||
[[Category: Rose | [[Category: Panepucci E]] | ||
[[Category: Rose JP]] | |||
[[Category: Waltersperger | [[Category: Waltersperger S]] | ||
[[Category: Wang | [[Category: Wang BC]] | ||
[[Category: Wang | [[Category: Wang M]] | ||
[[Category: Weinert | [[Category: Weinert T]] | ||
Latest revision as of 03:43, 28 December 2023
Crystal structure of hypothetical protein PF0907 from Pyrococcus furiosus solved by sulfur SAD using Swiss Light Source dataCrystal structure of hypothetical protein PF0907 from Pyrococcus furiosus solved by sulfur SAD using Swiss Light Source data
Structural highlights
FunctionPublication Abstract from PubMedWe describe a data collection method that uses a single crystal to solve X-ray structures by native SAD (single-wavelength anomalous diffraction). We solved the structures of 11 real-life examples, including a human membrane protein, a protein-DNA complex and a 266-kDa multiprotein-ligand complex, using this method. The data collection strategy is suitable for routine structure determination and can be implemented at most macromolecular crystallography synchrotron beamlines. Fast native-SAD phasing for routine macromolecular structure determination.,Weinert T, Olieric V, Waltersperger S, Panepucci E, Chen L, Zhang H, Zhou D, Rose J, Ebihara A, Kuramitsu S, Li D, Howe N, Schnapp G, Pautsch A, Bargsten K, Prota AE, Surana P, Kottur J, Nair DT, Basilico F, Cecatiello V, Pasqualato S, Boland A, Weichenrieder O, Wang B, Steinmetz MO, Caffrey M, Wang M Nat Methods. 2014 Dec 15. doi: 10.1038/nmeth.3211. PMID:25506719[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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