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==Crystal Structure of Antitermination Factors NusB and NusE in complex with dsRNA==
==Crystal Structure of Antitermination Factors NusB and NusE in complex with dsRNA==
<StructureSection load='3r2d' size='340' side='right' caption='[[3r2d]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
<StructureSection load='3r2d' size='340' side='right'caption='[[3r2d]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[3r2d]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3R2D FirstGlance]. <br>
<table><tr><td colspan='2'>[[3r2d]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Aquifex_aeolicus Aquifex aeolicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3R2D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3R2D FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.199&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3r2c|3r2c]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">aq_133, nusB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001]), aq_008, NUSE, rpsJ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3r2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r2d OCA], [https://pdbe.org/3r2d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3r2d RCSB], [https://www.ebi.ac.uk/pdbsum/3r2d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3r2d ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3r2d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3r2d OCA], [http://pdbe.org/3r2d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3r2d RCSB], [http://www.ebi.ac.uk/pdbsum/3r2d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3r2d ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/NUSB_AQUAE NUSB_AQUAE]] Involved in the transcription termination process (By similarity). [[http://www.uniprot.org/uniprot/RS10_AQUAE RS10_AQUAE]] Involved in the binding of tRNA to the ribosomes.[HAMAP-Rule:MF_00508]
[https://www.uniprot.org/uniprot/NUSB_AQUAE NUSB_AQUAE] Involved in the transcription termination process (By similarity).
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
Processive transcription antitermination requires the assembly of the complete antitermination complex, which is initiated by the formation of the ternary NusB-NusE-BoxA RNA complex. We have elucidated the crystal structure of this complex, demonstrating that the BoxA RNA is composed of 8 nt that are recognized by the NusB-NusE heterodimer. Functional biologic and biophysical data support the structural observations and establish the relative significance of key protein-protein and protein-RNA interactions. Further crystallographic investigation of a NusB-NusE-dsRNA complex reveals a heretofore unobserved dsRNA binding site contiguous with the BoxA binding site. We propose that the observed dsRNA represents BoxB RNA, as both single-stranded BoxA and double-stranded BoxB components are present in the classical lambda antitermination site. Combining these data with known interactions amongst antitermination factors suggests a specific model for the assembly of the complete antitermination complex.


Structural basis for RNA recognition by NusB and NusE in the initiation of transcription antitermination.,Stagno JR, Altieri AS, Bubunenko M, Tarasov SG, Li J, Court DL, Byrd RA, Ji X Nucleic Acids Res. 2011 Jun 7. PMID:21652641<ref>PMID:21652641</ref>
==See Also==
 
*[[Ribosomal protein S10|Ribosomal protein S10]]
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 3r2d" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Aquifex aeolicus huber and stetter 2001]]
[[Category: Aquifex aeolicus]]
[[Category: Ji, X]]
[[Category: Large Structures]]
[[Category: Stagno, J R]]
[[Category: Ji X]]
[[Category: Cross species nusb-nuse-rna interaction]]
[[Category: Stagno JR]]
[[Category: Gene regulation]]
[[Category: Protein-rna interaction]]
[[Category: Transcription elongation]]
[[Category: Transcription-rna complex]]

Latest revision as of 15:07, 14 March 2024

Crystal Structure of Antitermination Factors NusB and NusE in complex with dsRNACrystal Structure of Antitermination Factors NusB and NusE in complex with dsRNA

Structural highlights

3r2d is a 6 chain structure with sequence from Aquifex aeolicus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.199Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

NUSB_AQUAE Involved in the transcription termination process (By similarity).

See Also

3r2d, resolution 2.20Å

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