1nat: Difference between revisions

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 ==CRYSTAL STRUCTURE OF SPOOF FROM BACILLUS SUBTILIS==
-<StructureSection load='1nat' size='340' side='right' caption='[[1nat]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
+<StructureSection load='1nat' size='340' side='right'caption='[[1nat]], [[Resolution|resolution]] 2.45&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1nat]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_globigii"_migula_1900 "bacillus globigii" migula 1900]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NAT OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1NAT FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1nat]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_subtilis Bacillus subtilis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NAT OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1NAT FirstGlance]. <br>
-</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">SPO0F ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1423 "Bacillus globigii" Migula 1900])</td></tr>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.45&#8491;</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nat OCA], [http://pdbe.org/1nat PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1nat RCSB], [http://www.ebi.ac.uk/pdbsum/1nat PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1nat ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1nat FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nat OCA], [https://pdbe.org/1nat PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1nat RCSB], [https://www.ebi.ac.uk/pdbsum/1nat PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1nat ProSAT]</span></td></tr>
 </table>
 == Function ==
-[[http://www.uniprot.org/uniprot/SP0F_BACSU SP0F_BACSU]] Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation.
+[https://www.uniprot.org/uniprot/SP0F_BACSU SP0F_BACSU] Key element in the phosphorelay regulating sporulation initiation. Phosphorylation of spo0B during sporulation initiation.
 == Evolutionary Conservation ==
 [[Image:Consurf_key_small.gif|200px|right]]
 Check<jmol>
    <jmolCheckbox>
-     <scriptWhenChecked>select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/na/1nat_consurf.spt"</scriptWhenChecked>
+     <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/na/1nat_consurf.spt"</scriptWhenChecked>
      <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
      <text>to colour the structure by Evolutionary Conservation</text>
@@ Line 19: / Line 19: @@
 </jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1nat ConSurf].
 <div style="clear:both"></div>
-<div style="background-color:#fffaf0;">
-== Publication Abstract from PubMed ==
-Spo0F is a secondary messenger in the "two-component" system controlling the sporulation of Bacillus subtilis. Spo0F, like the chemotaxis protein CheY, is a single-domain protein homologous to the N-terminal activator domain of the response regulators. We recently reported the crystal structure of a phosphatase-resistant mutant Y13S of Spo0F with Ca2+ bound in the active site. The crystal structure of wild-type Spo0F in the absence of a metal ion is presented here. A comparison of the two structures reveals that the cation induces significant changes in the active site. In the present wild-type structure, the carboxylate of Asp11 points away from the center of the active site, whereas when coordinated to the Ca2+, as in the earlier structure, it points toward the active site. In addition, Asp54, the site of phosphorylation, is blocked by a salt bridge interaction of an Arg side chain from a neighboring molecule. From fluorescence quenching studies with Spo0F Y13W, we found that only the amino acid Arg binds to Spo0F in a saturable manner (Kd = 15 mM). This observation suggests that a small molecule with a shape complementary to the active site and having a guanidinium group might inhibit phosphotransfer between response regulators and their cognate histidine kinases.
-A response regulatory protein with the site of phosphorylation blocked by an arginine interaction: crystal structure of Spo0F from Bacillus subtilis.,Madhusudan M, Zapf J, Hoch JA, Whiteley JM, Xuong NH, Varughese KI Biochemistry. 1997 Oct 21;36(42):12739-45. PMID:9335530<ref>PMID:9335530</ref>
-From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-</div>
-<div class="pdbe-citations 1nat" style="background-color:#fffaf0;"></div>
 ==See Also==
-*[[Phosphotransferase|Phosphotransferase]]
+*[[Phosphotransferase 3D structures|Phosphotransferase 3D structures]]
-== References ==
+*[[Response regulator 3D structure|Response regulator 3D structure]]
-<references/>
 __TOC__
 </StructureSection>
-[[Category: Bacillus globigii migula 1900]]
+[[Category: Bacillus subtilis]]
-[[Category: Hoch, J A]]
+[[Category: Large Structures]]
+[[Category: Hoch JA]]
 [[Category: Madhusudan]]
-[[Category: Varughese, K I]]
+[[Category: Varughese KI]]
-[[Category: Whiteley, J M]]
+[[Category: Whiteley JM]]
-[[Category: Xuong, N H]]
+[[Category: Xuong NH]]
-[[Category: Zapf, J]]
+[[Category: Zapf J]]
-[[Category: Aspartate pocket]]
-[[Category: Regulatory protein]]
-[[Category: Sporulation response regulator]]
-[[Category: Two component system]]