6am3: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 6am3 is ON HOLD  until Paper Publication
+==Regulator of G protein signaling (RGS) 17 in complex with Ca2+==
+<StructureSection load='6am3' size='340' side='right'caption='[[6am3]], [[Resolution|resolution]] 1.53&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[6am3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AM3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AM3 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.53&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=PG4:TETRAETHYLENE+GLYCOL'>PG4</scene>, <scene name='pdbligand=PGE:TRIETHYLENE+GLYCOL'>PGE</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6am3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6am3 OCA], [https://pdbe.org/6am3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6am3 RCSB], [https://www.ebi.ac.uk/pdbsum/6am3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6am3 ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/RGS17_HUMAN RGS17_HUMAN] Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins (By similarity).
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Regulator of G protein signaling (RGS) proteins are negative regulators of G protein-coupled receptor (GPCR) signaling through their ability to act as GTPase-activating proteins (GAPs) for activated Galpha subunits. Members of the RZ subfamily of RGS proteins bind to activated Galphao, Galphaz, and Galphai1-3 proteins in the nervous system and thereby inhibit downstream pathways, including those involved in Ca(2+)-dependent signaling. In contrast to other RGS proteins, little is known about RZ subfamily structure and regulation. Herein, we present the 1.5-A crystal structure of RGS17, the most complete and highest-resolution structure of an RZ subfamily member to date. RGS17 cocrystallized with Ca(2+) bound to conserved positions on the predicted Galpha-binding surface of the protein. Using NMR chemical shift perturbations, we confirmed that Ca(2+) binds in solution to the same site. Furthermore, RGS17 had greater than 55-fold higher affinity for Ca(2+) than for Mg(2+) Finally, we found that Ca(2+) promotes interactions between RGS17 and activated Galpha and decreases the Km for GTP hydrolysis, potentially by altering the binding mechanism between these proteins. Taken together, these findings suggest that Ca(2+) positively regulates RGS17, which may represent a general mechanism by which increased Ca(2+) concentration promotes the GAP activity of the RZ subfamily, leading to RZ-mediated inhibition of Ca(2+) signaling.
-Authors:
+High-resolution structure of RGS17 suggests a role for Ca(2+) in promoting the GTPase-activating protein activity by RZ subfamily members.,Sieng M, Hayes MP, O'Brien JB, Andrew Fowler C, Houtman JC, Roman DL, Lyon AM J Biol Chem. 2019 May 17;294(20):8148-8160. doi: 10.1074/jbc.RA118.006059. Epub, 2019 Apr 2. PMID:30940727<ref>PMID:30940727</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 6am3" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Regulator of G-protein signaling 3D structures|Regulator of G-protein signaling 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Homo sapiens]]
+[[Category: Large Structures]]
+[[Category: Lyon AM]]
+[[Category: Sieng M]]