5y01: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 5y01 is ON HOLD  until Paper Publication
+==Acid-tolerant monomeric GFP, Gamillus, non-fluorescence (OFF) state==
+<StructureSection load='5y01' size='340' side='right'caption='[[5y01]], [[Resolution|resolution]] 2.65&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5y01]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Olindias Olindias]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Y01 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5Y01 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.65&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRQ:[2-(3-CARBAMOYL-1-IMINO-PROPYL)-4-(4-HYDROXY-BENZYLIDENE)-5-OXO-4,5-DIHYDRO-IMIDAZOL-1-YL]-ACETIC+ACID'>CRQ</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5y01 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5y01 OCA], [https://pdbe.org/5y01 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5y01 RCSB], [https://www.ebi.ac.uk/pdbsum/5y01 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5y01 ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+The fluorescent protein (FP) color palette has greatly contributed to the visualization of molecular and cellular processes. However, most FPs lose fluorescence at a pH lower than their neutral pKa ( approximately 6), and this has hampered their application in acidic organelles (pH approximately 4.5-6.0). Currently, several cyan- and red-colored acid-tolerant FPs are available; however, there are few reports of acid-tolerant green FPs (GFPs) that are practically applicable to bioimaging. Here, we developed the acid-tolerant monomeric GFP "Gamillus" from the jellyfish Olindias formosa, with excellent brightness, maturation speed, and photostability. Results from X-ray crystallography and point mutagenesis suggest that across a broad pH range the acid tolerance is attributed to stabilization of deprotonation in the chromophore phenyl ring by forming a unique trans configuration. We demonstrate that Gamillus can serve as a molecular tag suitable for imaging in acidic organelles through autophagy-mediated molecular tracking to lysosomes.
-Authors: Nakashima, R., Sakurai, K., Shinoda, H., Matsuda, T., Nagai, T.
+Acid-Tolerant Monomeric GFP from Olindias formosa.,Shinoda H, Ma Y, Nakashima R, Sakurai K, Matsuda T, Nagai T Cell Chem Biol. 2017 Dec 23. pii: S2451-9456(17)30432-4. doi:, 10.1016/j.chembiol.2017.12.005. PMID:29290624<ref>PMID:29290624</ref>
-Description: Acid-tolerant monomeric GFP, Gamillus, non-fluorescence (OFF) state
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Shinoda, H]]
+<div class="pdbe-citations 5y01" style="background-color:#fffaf0;"></div>
-[[Category: Matsuda, T]]
-[[Category: Sakurai, K]]
+==See Also==
-[[Category: Nakashima, R]]
+*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
-[[Category: Nagai, T]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Olindias]]
+[[Category: Matsuda T]]
+[[Category: Nagai T]]
+[[Category: Nakashima R]]
+[[Category: Sakurai K]]
+[[Category: Shinoda H]]