5xtu: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 5xtu is ON HOLD  until Paper Publication
+==Crystal Structure of GDSL Esterase of Photobacterium sp. J15==
+<StructureSection load='5xtu' size='340' side='right'caption='[[5xtu]], [[Resolution|resolution]] 1.38&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5xtu]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Photobacterium_johormaris Photobacterium johormaris]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XTU OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XTU FirstGlance]. <br>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CAC:CACODYLATE+ION'>CAC</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene>, <scene name='pdbligand=PO4:PHOSPHATE+ION'>PO4</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xtu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xtu OCA], [http://pdbe.org/5xtu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xtu RCSB], [http://www.ebi.ac.uk/pdbsum/5xtu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xtu ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+GDSL esterase J15 (EstJ15) is a member of Family II of lipolytic enzyme. The enzyme was further classified in subgroup SGNH hydrolase due to the presence of highly conserve motif, Ser-Gly-Asn-His in four conserved blocks I, II, III, and V, respectively. X-ray quality crystal of EstJ15 was obtained from optimized formulation containing 0.10M ammonium sulphate, 0.15M sodium cacodylate trihydrate pH6.5, and 20% PEG 8000. The crystal structure of EstJ15 was solved at 1.38A with one molecule per asymmetric unit. The structure exhibits alpha/beta hydrolase fold and shared low amino acid sequence identity of 23% with the passenger domain of the autotransporter EstA of Pseudomonas aeruginosa. The active site is located at the centre of the structure, formed a narrow tunnel that hinder long substrates to be catalysed which was proven by the protein-ligand docking analysis. This study facilitates the understanding of high substrate specificity of EstJ15 and provide insights on its catalytic mechanism.
-Authors: Mazlan, S.N.H.S., Leow, T.C., Jonet, A.
+Crystallization and structure elucidation of GDSL esterase of Photobacterium sp. J15.,Mazlan SNHS, Ali MSM, Rahman RNZRA, Sabri S, Jonet MA, Leow TC Int J Biol Macromol. 2018 Nov;119:1188-1194. doi: 10.1016/j.ijbiomac.2018.08.022., Epub 2018 Aug 10. PMID:30102982<ref>PMID:30102982</ref>
-Description: Crystal Structure of GDSL Esterase of Photobacterium sp. J15
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Leow, T.C]]
+<div class="pdbe-citations 5xtu" style="background-color:#fffaf0;"></div>
-[[Category: Mazlan, S.N.H.S]]
+== References ==
-[[Category: Jonet, A]]
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Photobacterium johormaris]]
+[[Category: Ali, M S.M]]
+[[Category: Jonet, M A]]
+[[Category: Leow, T C]]
+[[Category: Mazlan, S N.H S]]
+[[Category: Rahman, R N.Z R.A]]
+[[Category: Esterase]]
+[[Category: Gdsl]]
+[[Category: Hydrolase]]
+[[Category: Photobacterium]]
+[[Category: Sgnh]]