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[[Image:1xm5.jpg|left|200px]]


{{Structure
==Crystal structure of metal-dependent hydrolase ybeY from E. coli, Pfam UPF0054==
|PDB= 1xm5 |SIZE=350|CAPTION= <scene name='initialview01'>1xm5</scene>, resolution 2.7&Aring;
<StructureSection load='1xm5' size='340' side='right'caption='[[1xm5]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
|SITE=  
== Structural highlights ==
|LIGAND= <scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene>
<table><tr><td colspan='2'>[[1xm5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XM5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1XM5 FirstGlance]. <br>
|ACTIVITY=
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7&#8491;</td></tr>
|GENE= ybeY ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli])
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NI:NICKEL+(II)+ION'>NI</scene></td></tr>
|DOMAIN=<span class='plainlinks'>[http://www.ncbi.nlm.nih.gov/Structure/cdd/cddsrv.cgi?uid=PRK00016 PRK00016]</span>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1xm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xm5 OCA], [https://pdbe.org/1xm5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1xm5 RCSB], [https://www.ebi.ac.uk/pdbsum/1xm5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1xm5 ProSAT], [https://www.topsan.org/Proteins/NYSGXRC/1xm5 TOPSAN]</span></td></tr>
|RELATEDENTRY=
</table>
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xm5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xm5 OCA], [http://www.ebi.ac.uk/pdbsum/1xm5 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1xm5 RCSB]</span>
== Function ==
}}
[https://www.uniprot.org/uniprot/YBEY_ECOLI YBEY_ECOLI] Single strand-specific metallo-endoribonuclease involved in late-stage 70S ribosome quality control and in maturation of the 3' terminus of the 16S rRNA. Acts together with the RNase R to eliminate defective 70S ribosomes, but not properly matured 70S ribosomes or individual subunits, by a process mediated specifically by the 30S ribosomal subunit. Involved in the processing of 16S, 23S and 5S rRNAs, with a particularly strong effect on maturation at both the 5'-and 3'-ends of 16S rRNA as well as maturation of the 5'-end of 23S and 5S rRNAs.<ref>PMID:20639334</ref> <ref>PMID:20807199</ref> <ref>PMID:23273979</ref> <ref>PMID:16511207</ref>
 
== Evolutionary Conservation ==
'''Crystal structure of metal-dependent hydrolase ybeY from E. coli, Pfam UPF0054'''
[[Image:Consurf_key_small.gif|200px|right]]
 
Check<jmol>
 
  <jmolCheckbox>
==Overview==
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/xm/1xm5_consurf.spt"</scriptWhenChecked>
The three-dimensional crystallographic structure of the ybeY protein from Escherichia coli (SwissProt entry P77385) is reported at 2.7 A resolution. YbeY is a hypothetical protein that belongs to the UPF0054 family. The structure reveals that the protein binds a metal ion in a tetrahedral geometry. Three coordination sites are provided by histidine residues, while the fourth might be a water molecule that is not seen in the diffraction map because of its relatively low resolution. X-ray fluorescence analysis of the purified protein suggests that the metal is a nickel ion. The structure of ybeY and its sequence similarity to a number of predicted metal-dependent hydrolases provides a functional assignment for this protein family. The figures and tables of this paper were prepared using semi-automated tools, termed the Autopublish server, developed by the New York Structural GenomiX Research Consortium, with the goal of facilitating the rapid publication of crystallographic structures that emanate from worldwide Structural Genomics efforts, including the NIH-funded Protein Structure Initiative.
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
 
    <text>to colour the structure by Evolutionary Conservation</text>
==About this Structure==
  </jmolCheckbox>
1XM5 is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XM5 OCA].  
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1xm5 ConSurf].
 
<div style="clear:both"></div>
==Reference==
== References ==
The ybeY protein from Escherichia coli is a metalloprotein., Zhan C, Fedorov EV, Shi W, Ramagopal UA, Thirumuruhan R, Manjasetty BA, Almo SC, Fiser A, Chance MR, Fedorov AA, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Nov 1;61(Pt, 11):959-63. Epub 2005 Oct 20. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16511207 16511207]
<references/>
__TOC__
</StructureSection>
[[Category: Escherichia coli]]
[[Category: Escherichia coli]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Almo, S C.]]
[[Category: Almo SC]]
[[Category: Burley, S K.]]
[[Category: Burley SK]]
[[Category: Fedorov, A A.]]
[[Category: Fedorov AA]]
[[Category: Fedorov, E V.]]
[[Category: Fedorov EV]]
[[Category: NYSGXRC, New York Structural GenomiX Research Consortium.]]
[[Category: Ramagopal UA]]
[[Category: Ramagopal, U A.]]
[[Category: Shi W]]
[[Category: Shi, W.]]
[[Category: Thirumuruhan R]]
[[Category: Thirumuruhan, R.]]
[[Category: new york structural genomix research consortium]]
[[Category: nysgxrc target t842]]
[[Category: pfam family upf0054]]
[[Category: protein structure initiative]]
[[Category: psi]]
[[Category: structural genomic]]
 
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