5v8e: Difference between revisions

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-'''Unreleased structure'''
-The entry 5v8e is ON HOLD  until Paper Publication
+==Structure of Bacillus cereus PatB1==
+<StructureSection load='5v8e' size='340' side='right'caption='[[5v8e]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5v8e]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_cereus_ATCC_10987 Bacillus cereus ATCC 10987]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5V8E OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5V8E FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CIT:CITRIC+ACID'>CIT</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PEG:DI(HYDROXYETHYL)ETHER'>PEG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5v8e FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5v8e OCA], [https://pdbe.org/5v8e PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5v8e RCSB], [https://www.ebi.ac.uk/pdbsum/5v8e PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5v8e ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/Q73CU0_BACC1 Q73CU0_BACC1]
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+O-Acetylation of the secondary cell wall polysaccharides (SCWP) of the Bacillus cereus group of pathogens, which includes Bacillus anthracis, is essential for the proper attachment of surface-layer (S-layer) proteins to their cell walls. Using a variety of pseudosubstrates and a chemically synthesized analog of SCWP, we report here the identification of PatB1 as a SCWP O-acetyltransferase in Bacillus cereus. Additionally, we report the crystal structure of PatB1, which provides detailed insights into the mechanism of this enzyme and defines a novel subfamily of the SGNH family of esterases and lipases. We propose a model for the O-acetylation of SCWP requiring the translocation of acetyl groups from a cytoplasmic source across the plasma membrane by PatA1 and PatA2 for their transfer to SCWP by PatB1.
-Authors: Sychantha, D., Little, D.J., Chapman, R.N., Boons, G.J., Robinson, H., Howell, P.L., Clarke, A.J.
+PatB1 is an O-acetyltransferase that decorates secondary cell wall polysaccharides.,Sychantha D, Little DJ, Chapman RN, Boons GJ, Robinson H, Howell PL, Clarke AJ Nat Chem Biol. 2017 Oct 30. pii: nchembio.2509. doi: 10.1038/nchembio.2509. PMID:29083419<ref>PMID:29083419</ref>
-Description: Structure of Bacillus cereus PatB1
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Clarke, A.J]]
+<div class="pdbe-citations 5v8e" style="background-color:#fffaf0;"></div>
-[[Category: Robinson, H]]
+== References ==
-[[Category: Sychantha, D]]
+<references/>
-[[Category: Little, D.J]]
+__TOC__
-[[Category: Howell, P.L]]
+</StructureSection>
-[[Category: Boons, G.J]]
+[[Category: Bacillus cereus ATCC 10987]]
-[[Category: Chapman, R.N]]
+[[Category: Large Structures]]
+[[Category: Boons GJ]]
+[[Category: Chapman RN]]
+[[Category: Clarke AJ]]
+[[Category: Howell PL]]
+[[Category: Little DJ]]
+[[Category: Robinson H]]
+[[Category: Sychantha D]]