5o7m: Difference between revisions

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-'''Unreleased structure'''
-The entry 5o7m is ON HOLD
+==Single-shot pink beam serial crystallography: Phycocyanin (One chip, chip_1)==
+<StructureSection load='5o7m' size='340' side='right'caption='[[5o7m]], [[Resolution|resolution]] 2.46&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5o7m]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermosynechococcus_vestitus_BP-1 Thermosynechococcus vestitus BP-1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5O7M OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5O7M FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.46&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CYC:PHYCOCYANOBILIN'>CYC</scene>, <scene name='pdbligand=MEN:N-METHYL+ASPARAGINE'>MEN</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5o7m FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5o7m OCA], [https://pdbe.org/5o7m PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5o7m RCSB], [https://www.ebi.ac.uk/pdbsum/5o7m PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5o7m ProSAT]</span></td></tr>
+</table>
+== Function ==
+[https://www.uniprot.org/uniprot/PHCB_THEVB PHCB_THEVB] Light-harvesting photosynthetic bile pigment-protein from the phycobiliprotein complex (phycobilisome, PBS). Phycocyanin is the major phycobiliprotein in the PBS rod.
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+Serial X-ray crystallography allows macromolecular structure determination at both X-ray free electron lasers (XFELs) and, more recently, synchrotron sources. The time resolution for serial synchrotron crystallography experiments has been limited to millisecond timescales with monochromatic beams. The polychromatic, "pink", beam provides a more than two orders of magnitude increased photon flux and hence allows accessing much shorter timescales in diffraction experiments at synchrotron sources. Here we report the structure determination of two different protein samples by merging pink-beam diffraction patterns from many crystals, each collected with a single 100 ps X-ray pulse exposure per crystal using a setup optimized for very low scattering background. In contrast to experiments with monochromatic radiation, data from only 50 crystals were required to obtain complete datasets. The high quality of the diffraction data highlights the potential of this method for studying irreversible reactions at sub-microsecond timescales using high-brightness X-ray facilities.
-Authors: Meents, A., Oberthuer, D., Lieske, J., Srajer, V., Sarrou, I.
+Pink-beam serial crystallography.,Meents A, Wiedorn MO, Srajer V, Henning R, Sarrou I, Bergtholdt J, Barthelmess M, Reinke PYA, Dierksmeyer D, Tolstikova A, Schaible S, Messerschmidt M, Ogata CM, Kissick DJ, Taft MH, Manstein DJ, Lieske J, Oberthuer D, Fischetti RF, Chapman HN Nat Commun. 2017 Nov 3;8(1):1281. doi: 10.1038/s41467-017-01417-3. PMID:29097720<ref>PMID:29097720</ref>
-Description: Single-shot pink beam serial crystallography: Phycocyanin (One chip, chip_1)
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
-[[Category: Oberthuer, D]]
+<div class="pdbe-citations 5o7m" style="background-color:#fffaf0;"></div>
-[[Category: Srajer, V]]
-[[Category: Lieske, J]]
+==See Also==
-[[Category: Sarrou, I]]
+*[[Phycocyanin|Phycocyanin]]
-[[Category: Meents, A]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Thermosynechococcus vestitus BP-1]]
+[[Category: Lieske J]]
+[[Category: Meents A]]
+[[Category: Oberthuer D]]
+[[Category: Sarrou I]]
+[[Category: Srajer V]]