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==RsgA-GDPNP bound to the 30S ribosomal subunit (RsgA assembly intermediate with uS3)== | |||
<SX load='5no4' size='340' side='right' viewer='molstar' caption='[[5no4]], [[Resolution|resolution]] 5.16Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5no4]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli_K-12 Escherichia coli K-12]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5NO4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5NO4 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution|Resolution]] 5.16Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=2MG:2N-METHYLGUANOSINE-5-MONOPHOSPHATE'>2MG</scene>, <scene name='pdbligand=4OC:4N,O2-METHYLCYTIDINE-5-MONOPHOSPHATE'>4OC</scene>, <scene name='pdbligand=5MC:5-METHYLCYTIDINE-5-MONOPHOSPHATE'>5MC</scene>, <scene name='pdbligand=G7M:N7-METHYL-GUANOSINE-5-MONOPHOSPHATE'>G7M</scene>, <scene name='pdbligand=GNP:PHOSPHOAMINOPHOSPHONIC+ACID-GUANYLATE+ESTER'>GNP</scene>, <scene name='pdbligand=MA6:6N-DIMETHYLADENOSINE-5-MONOPHOSHATE'>MA6</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PSU:PSEUDOURIDINE-5-MONOPHOSPHATE'>PSU</scene>, <scene name='pdbligand=UR3:3-METHYLURIDINE-5-MONOPHOSHATE'>UR3</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5no4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5no4 OCA], [https://pdbe.org/5no4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5no4 RCSB], [https://www.ebi.ac.uk/pdbsum/5no4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5no4 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/RS3_ECOLI RS3_ECOLI] Binds the lower part of the 30S subunit head. Binds mRNA in the 70S ribosome, positioning it for translation (By similarity).<ref>PMID:15652481</ref> Plays a role in mRNA unwinding by the ribosome, possibly by forming part of a processivity clamp.<ref>PMID:15652481</ref> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
During 30S ribosomal subunit biogenesis, assembly factors are believed to prevent accumulation of misfolded intermediate states of low free energy that slowly convert into mature 30S subunits, namely, kinetically trapped particles. Among the assembly factors, the circularly permuted GTPase, RsgA, plays a crucial role in the maturation of the 30S decoding center. Here, directed hydroxyl radical probing and single particle cryo-EM are employed to elucidate RsgA's mechanism of action. Our results show that RsgA destabilizes the 30S structure, including late binding r-proteins, providing a structural basis for avoiding kinetically trapped assembly intermediates. Moreover, RsgA exploits its distinct GTPase pocket and specific interactions with the 30S to coordinate GTPase activation with the maturation state of the 30S subunit. This coordination validates the architecture of the decoding center and facilitates the timely release of RsgA to control the progression of 30S biogenesis. | |||
RsgA couples the maturation state of the 30S ribosomal decoding center to activation of its GTPase pocket.,Lopez-Alonso JP, Kaminishi T, Kikuchi T, Hirata Y, Iturrioz I, Dhimole N, Schedlbauer A, Hase Y, Goto S, Kurita D, Muto A, Zhou S, Naoe C, Mills DJ, Gil-Carton D, Takemoto C, Himeno H, Fucini P, Connell SR Nucleic Acids Res. 2017 May 8. doi: 10.1093/nar/gkx324. PMID:28482099<ref>PMID:28482099</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
<div class="pdbe-citations 5no4" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Ribosome 3D structures|Ribosome 3D structures]] | |||
== References == | |||
<references/> | |||
__TOC__ | |||
</SX> | |||
[[Category: Escherichia coli K-12]] | |||
[[Category: Large Structures]] | |||
[[Category: Connell SR]] | |||
[[Category: Dhimole N]] | |||
[[Category: Fucini P]] | |||
[[Category: Gil-Carton D]] | |||
[[Category: Goto S]] | |||
[[Category: Hase Y]] | |||
[[Category: Himeno H]] | |||
[[Category: Hirata Y]] | |||
[[Category: Iturrioz I]] | |||
[[Category: Kaminishi T]] | |||
[[Category: Kikuchi T]] | |||
[[Category: Kurita D]] | |||
[[Category: Lopez-Alonso JP]] | |||
[[Category: Mills DJ]] | |||
[[Category: Muto A]] | |||
[[Category: Naoe C]] | |||
[[Category: Schedlbauer A]] | |||
[[Category: Takemoto C]] | |||
[[Category: Zhou S]] | |||
Latest revision as of 16:13, 15 November 2023
RsgA-GDPNP bound to the 30S ribosomal subunit (RsgA assembly intermediate with uS3)RsgA-GDPNP bound to the 30S ribosomal subunit (RsgA assembly intermediate with uS3)
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