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==Structure of T4Pnkp 3' phosphatase covalently bound to BeF3==
==Structure of T4Pnkp 3' phosphatase covalently bound to BeF3==
<StructureSection load='5uj0' size='340' side='right' caption='[[5uj0]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
<StructureSection load='5uj0' size='340' side='right'caption='[[5uj0]], [[Resolution|resolution]] 2.30&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[5uj0]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UJ0 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5UJ0 FirstGlance]. <br>
<table><tr><td colspan='2'>[[5uj0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UJ0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UJ0 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.3&#8491;</td></tr>
<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=BFD:ASPARTATE+BERYLLIUM+TRIFLUORIDE'>BFD</scene></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BFD:ASPARTATE+BERYLLIUM+TRIFLUORIDE'>BFD</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5uj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uj0 OCA], [http://pdbe.org/5uj0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5uj0 RCSB], [http://www.ebi.ac.uk/pdbsum/5uj0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5uj0 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uj0 OCA], [https://pdbe.org/5uj0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uj0 RCSB], [https://www.ebi.ac.uk/pdbsum/5uj0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uj0 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/KIPN_BPT4 KIPN_BPT4]] Acts as a 5'-hydroxyl kinase, a 3'-phosphatase and a 2',3'-cyclic phosphodiesterase. Catalyzes the transfer of the terminal phosphate of ATP to the 5'-hydroxyl termini of ribo- and deoxyribonucleotides. In the presence of ADP the enzyme also catalyzes an exchange reaction. In the exchange reaction, an excess ADP causes the enzyme to transfer the 5' terminal phosphate from phosphorylated DNA to ADP. These activities modify the ends of nicked tRNA generated by a bacterial response to infection and facilitate repair by T4 RNA ligase.  
[https://www.uniprot.org/uniprot/KIPN_BPT4 KIPN_BPT4] Acts as a 5'-hydroxyl kinase, a 3'-phosphatase and a 2',3'-cyclic phosphodiesterase. Catalyzes the transfer of the terminal phosphate of ATP to the 5'-hydroxyl termini of ribo- and deoxyribonucleotides. In the presence of ADP the enzyme also catalyzes an exchange reaction. In the exchange reaction, an excess ADP causes the enzyme to transfer the 5' terminal phosphate from phosphorylated DNA to ADP. These activities modify the ends of nicked tRNA generated by a bacterial response to infection and facilitate repair by T4 RNA ligase.
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Chatterjee, D]]
[[Category: Escherichia virus T4]]
[[Category: Shuman, S]]
[[Category: Large Structures]]
[[Category: Bef3-bound]]
[[Category: Chatterjee D]]
[[Category: Beryllium]]
[[Category: Shuman S]]
[[Category: Hydrolase]]
[[Category: Phosphatase]]
[[Category: Pnkp]]
[[Category: Transferase]]

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