5mkk: Difference between revisions
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==Crystal structure of the heterodimeric ABC transporter TmrAB, a homolog of the antigen translocation complex TAP== | ==Crystal structure of the heterodimeric ABC transporter TmrAB, a homolog of the antigen translocation complex TAP== | ||
<StructureSection load='5mkk' size='340' side='right' caption='[[5mkk]], [[Resolution|resolution]] 2.70Å' scene=''> | <StructureSection load='5mkk' size='340' side='right'caption='[[5mkk]], [[Resolution|resolution]] 2.70Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5mkk]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MKK OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[5mkk]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Thermus_thermophilus_HB27 Thermus thermophilus HB27]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5MKK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5MKK FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.7Å</td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5mkk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5mkk OCA], [https://pdbe.org/5mkk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5mkk RCSB], [https://www.ebi.ac.uk/pdbsum/5mkk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5mkk ProSAT]</span></td></tr> | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q72J05_THET2 Q72J05_THET2] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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</div> | </div> | ||
<div class="pdbe-citations 5mkk" style="background-color:#fffaf0;"></div> | <div class="pdbe-citations 5mkk" style="background-color:#fffaf0;"></div> | ||
==See Also== | |||
*[[ABC transporter 3D structures|ABC transporter 3D structures]] | |||
== References == | == References == | ||
<references/> | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Diederichs | [[Category: Large Structures]] | ||
[[Category: Noell | [[Category: Thermus thermophilus HB27]] | ||
[[Category: Olieric | [[Category: Diederichs K]] | ||
[[Category: Pos | [[Category: Noell A]] | ||
[[Category: Stroud | [[Category: Olieric V]] | ||
[[Category: Tampe | [[Category: Pos KM]] | ||
[[Category: Thomas | [[Category: Stroud RM]] | ||
[[Category: Tomasiak | [[Category: Tampe R]] | ||
[[Category: Wang | [[Category: Thomas C]] | ||
[[Category: Tomasiak TM]] | |||
[[Category: Wang M]] | |||
Latest revision as of 21:48, 1 November 2023
Crystal structure of the heterodimeric ABC transporter TmrAB, a homolog of the antigen translocation complex TAPCrystal structure of the heterodimeric ABC transporter TmrAB, a homolog of the antigen translocation complex TAP
Structural highlights
FunctionPublication Abstract from PubMedABC transporters form one of the largest protein superfamilies in all domains of life, catalyzing the movement of diverse substrates across membranes. In this key position, ABC transporters can mediate multidrug resistance in cancer therapy and their dysfunction is linked to various diseases. Here, we describe the 2.7-A X-ray structure of heterodimeric Thermus thermophilus multidrug resistance proteins A and B (TmrAB), which not only shares structural homology with the antigen translocation complex TAP, but is also able to restore antigen processing in human TAP-deficient cells. TmrAB exhibits a broad peptide specificity and can concentrate substrates several thousandfold, using only one single active ATP-binding site. In our structure, TmrAB adopts an asymmetric inward-facing state, and we show that the C-terminal helices, arranged in a zipper-like fashion, play a crucial role in guiding the conformational changes associated with substrate transport. In conclusion, TmrAB can be regarded as a model system for asymmetric ABC exporters in general, and for TAP in particular. Crystal structure and mechanistic basis of a functional homolog of the antigen transporter TAP.,Noll A, Thomas C, Herbring V, Zollmann T, Barth K, Mehdipour AR, Tomasiak TM, Bruchert S, Joseph B, Abele R, Olieric V, Wang M, Diederichs K, Hummer G, Stroud RM, Pos KM, Tampe R Proc Natl Acad Sci U S A. 2017 Jan 9. pii: 201620009. doi:, 10.1073/pnas.1620009114. PMID:28069938[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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