5uf4: Difference between revisions

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OCA

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-'''Unreleased structure'''
-The entry 5uf4 is ON HOLD
+==Crystal Structure of Variable Lymphocyte Receptor (VLR) O13 with LNnT bound==
+<StructureSection load='5uf4' size='340' side='right'caption='[[5uf4]], [[Resolution|resolution]] 2.04&Aring;' scene=''>
+== Structural highlights ==
+<table><tr><td colspan='2'>[[5uf4]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Petromyzon_marinus Petromyzon marinus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UF4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UF4 FirstGlance]. <br>
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.04&#8491;</td></tr>
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GAL:BETA-D-GALACTOSE'>GAL</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uf4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uf4 OCA], [https://pdbe.org/5uf4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uf4 RCSB], [https://www.ebi.ac.uk/pdbsum/5uf4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uf4 ProSAT]</span></td></tr>
+</table>
+<div style="background-color:#fffaf0;">
+== Publication Abstract from PubMed ==
+High-quality reagents to study and detect glycans with high specificity for research and clinical applications are severely lacking. Here, we structurally and functionally characterize several variable lymphocyte receptor (VLR)-based antibodies from lampreys immunized with O erythrocytes that specifically recognize the blood group H-trisaccharide type II antigen. Glycan microarray analysis and biophysical data reveal that these VLRs exhibit greater specificity for H-trisaccharide compared with the plant lectin UEA-1, which is widely used in blood typing. Among these antibodies, O13 exhibits superior specificity for H-trisaccharide, the basis for which is revealed by comparative analysis of high-resolution VLR:glycan crystal structures. Using a structure-guided approach, we designed an O13 mutant with further enhanced specificity for H-trisaccharide. These insights into glycan recognition by VLRs suggest that lampreys can produce highly specific glycan antibodies, and are a valuable resource for the production of next-generation glycan reagents for biological and biomedical research and as diagnostics and therapeutics.
-Authors:
+Structural Insights into VLR Fine Specificity for Blood Group Carbohydrates.,Collins BC, Gunn RJ, McKitrick TR, Cummings RD, Cooper MD, Herrin BR, Wilson IA Structure. 2017 Sep 27. pii: S0969-2126(17)30294-0. doi:, 10.1016/j.str.2017.09.003. PMID:28988747<ref>PMID:28988747</ref>
-Description:
+From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
-[[Category: Unreleased Structures]]
+</div>
+<div class="pdbe-citations 5uf4" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Variable lymphocyte receptor 3D structures|Variable lymphocyte receptor 3D structures]]
+== References ==
+<references/>
+__TOC__
+</StructureSection>
+[[Category: Large Structures]]
+[[Category: Petromyzon marinus]]
+[[Category: Collins BC]]
+[[Category: Cooper MD]]
+[[Category: Cummings RD]]
+[[Category: Gunn RJ]]
+[[Category: Herrin BR]]
+[[Category: McKitrick TR]]
+[[Category: Wilson IA]]