5ubi: Difference between revisions
No edit summary |
No edit summary |
||
| (3 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
The | ==Catalytic core domain of Adenosine triphosphate phosphoribosyltransferase from Campylobacter jejuni with bound PRPP== | ||
<StructureSection load='5ubi' size='340' side='right'caption='[[5ubi]], [[Resolution|resolution]] 2.14Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5ubi]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Campylobacter_jejuni_RM1221 Campylobacter jejuni RM1221]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UBI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UBI FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.14Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=PRP:ALPHA-PHOSPHORIBOSYLPYROPHOSPHORIC+ACID'>PRP</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ubi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ubi OCA], [https://pdbe.org/5ubi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ubi RCSB], [https://www.ebi.ac.uk/pdbsum/5ubi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ubi ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/HIS1_CAMJR HIS1_CAMJR] Catalyzes the condensation of ATP and 5-phosphoribose 1-diphosphate to form N'-(5'-phosphoribosyl)-ATP (PR-ATP). Has a crucial role in the pathway because the rate of histidine biosynthesis seems to be controlled primarily by regulation of HisG enzymatic activity. | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
ATP-phosphoribosyltransferase (ATP-PRT) catalyses the first committed step of histidine biosynthesis in plants and micro-organisms. Two forms of ATP-PRT have been reported, which differ in their molecular architecture and mechanism of allosteric regulation. The short-form ATP-PRT is a hetero-octamer, with four HisG chains that comprise only the catalytic domains and four separate chains of HisZ required for allosteric regulation by histidine. The long-form ATP-PRT is homohexameric, with each chain comprising two catalytic domains and a covalently linked regulatory domain that binds histidine as an allosteric inhibitor. Here, we describe a truncated long-form ATP-PRT from Campylobacter jejuni devoid of its regulatory domain ( Cje ATP-PRT(core)). Results showed that Cje ATP-PRT(core) is dimeric, exhibits attenuated catalytic activity, and is insensitive to histidine, indicating the covalently linked regulatory domain plays a role in both catalysis and regulation. Crystal structures were obtained for Cje ATP-PRT(core) in complex with both substrates, and for the first time, the complete product of the reaction. These structures reveal the key features of the active site, and provide insights into how substrates move into position during catalysis. | |||
A dimeric catalytic core relates the short and long forms of ATP-phosphoribosyltransferase.,Mittelstadt G, Jiao W, Livingstone E, Moggre GJ, Nazmi AR, Parker EJ Biochem J. 2017 Dec 5. pii: BCJ20170762. doi: 10.1042/BCJ20170762. PMID:29208762<ref>PMID:29208762</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5ubi" style="background-color:#fffaf0;"></div> | ||
[[Category: Jiao | |||
[[Category: | ==See Also== | ||
[[Category: Mittelstaedt | *[[ATP phosphoribosyl transferase 3D structures|ATP phosphoribosyl transferase 3D structures]] | ||
== References == | |||
<references/> | |||
__TOC__ | |||
</StructureSection> | |||
[[Category: Campylobacter jejuni RM1221]] | |||
[[Category: Large Structures]] | |||
[[Category: Jiao W]] | |||
[[Category: Livingstone EK]] | |||
[[Category: Mittelstaedt G]] | |||
[[Category: Parker EJ]] | |||