5uc0: Difference between revisions
New page: '''Unreleased structure''' The entry 5uc0 is ON HOLD Authors: Kim, Y., Bigelow, L., Endres, M., Babnigg, G., Crosson, S., Joachimiak, A., Chicago Center for Functional Annotation, Midwe... |
No edit summary |
||
| (6 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
==Crystal Structure of Beta-barrel-like, Uncharacterized Protein of COG5400 from Brucella abortus== | |||
<StructureSection load='5uc0' size='340' side='right'caption='[[5uc0]], [[Resolution|resolution]] 1.73Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5uc0]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Brucella_abortus_2308 Brucella abortus 2308]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5UC0 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5UC0 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.73Å</td></tr> | |||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1PE:PENTAETHYLENE+GLYCOL'>1PE</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5uc0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5uc0 OCA], [https://pdbe.org/5uc0 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5uc0 RCSB], [https://www.ebi.ac.uk/pdbsum/5uc0 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5uc0 ProSAT]</span></td></tr> | |||
</table> | |||
== Function == | |||
[https://www.uniprot.org/uniprot/Q2YQA6_BRUA2 Q2YQA6_BRUA2] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Molecular components of the Brucella abortus cell envelope play a major role in its ability to infect, colonize and survive inside mammalian host cells. In this study, we have defined a role for a conserved gene of unknown function in B. abortus envelope stress resistance and infection. Expression of this gene, which we name eipA, is directly activated by the essential cell cycle regulator, CtrA. eipA encodes a soluble periplasmic protein that adopts an unusual eight-stranded beta-barrel fold. Deletion of eipA attenuates replication and survival in macrophage and mouse infection models, and results in sensitivity to treatments that compromise the cell envelope integrity. Transposon disruption of genes required for LPS O-polysaccharide biosynthesis is synthetically lethal with eipA deletion. This genetic connection between O-polysaccharide and eipA is corroborated by our discovery that eipA is essential in Brucella ovis, a naturally rough species that harbors mutations in several genes required for O-polysaccharide production. Conditional depletion of eipA expression in B. ovis results in a cell chaining phenotype, providing evidence that eipA directly or indirectly influences cell division in Brucella. We conclude that EipA is a molecular determinant of Brucella virulence that functions to maintain cell envelope integrity and influences cell division. This article is protected by copyright. All rights reserved. | |||
Periplasmic protein EipA determines envelope stress resistance and virulence in Brucella abortus.,Herrou J, Willett JW, Fiebig A, Varesio LM, Czyz DM, Cheng JX, Ultee E, Briegel A, Bigelow L, Babnigg G, Kim Y, Crosson S Mol Microbiol. 2018 Dec 8. doi: 10.1111/mmi.14178. PMID:30536925<ref>PMID:30536925</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5uc0" style="background-color:#fffaf0;"></div> | ||
[[Category: Babnigg | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: | </StructureSection> | ||
[[Category: | [[Category: Brucella abortus 2308]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Babnigg G]] | |||
[[Category: Bigelow L]] | |||
[[Category: Crosson S]] | |||
[[Category: Endres M]] | |||
[[Category: Joachimiak A]] | |||
[[Category: Kim Y]] | |||
Latest revision as of 07:47, 21 November 2024
Crystal Structure of Beta-barrel-like, Uncharacterized Protein of COG5400 from Brucella abortusCrystal Structure of Beta-barrel-like, Uncharacterized Protein of COG5400 from Brucella abortus
Structural highlights
FunctionPublication Abstract from PubMedMolecular components of the Brucella abortus cell envelope play a major role in its ability to infect, colonize and survive inside mammalian host cells. In this study, we have defined a role for a conserved gene of unknown function in B. abortus envelope stress resistance and infection. Expression of this gene, which we name eipA, is directly activated by the essential cell cycle regulator, CtrA. eipA encodes a soluble periplasmic protein that adopts an unusual eight-stranded beta-barrel fold. Deletion of eipA attenuates replication and survival in macrophage and mouse infection models, and results in sensitivity to treatments that compromise the cell envelope integrity. Transposon disruption of genes required for LPS O-polysaccharide biosynthesis is synthetically lethal with eipA deletion. This genetic connection between O-polysaccharide and eipA is corroborated by our discovery that eipA is essential in Brucella ovis, a naturally rough species that harbors mutations in several genes required for O-polysaccharide production. Conditional depletion of eipA expression in B. ovis results in a cell chaining phenotype, providing evidence that eipA directly or indirectly influences cell division in Brucella. We conclude that EipA is a molecular determinant of Brucella virulence that functions to maintain cell envelope integrity and influences cell division. This article is protected by copyright. All rights reserved. Periplasmic protein EipA determines envelope stress resistance and virulence in Brucella abortus.,Herrou J, Willett JW, Fiebig A, Varesio LM, Czyz DM, Cheng JX, Ultee E, Briegel A, Bigelow L, Babnigg G, Kim Y, Crosson S Mol Microbiol. 2018 Dec 8. doi: 10.1111/mmi.14178. PMID:30536925[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
|
| ||||||||||||||||||