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| ==Dihydropteroate Synthase in complex with product== | | ==Dihydropteroate Synthase in complex with product== |
| <StructureSection load='3tya' size='340' side='right' caption='[[3tya]], [[Resolution|resolution]] 2.61Å' scene=''> | | <StructureSection load='3tya' size='340' side='right'caption='[[3tya]], [[Resolution|resolution]] 2.61Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3tya]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_anthracis_a2012 Bacillus anthracis a2012]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TYA OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3TYA FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3tya]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Bacillus_anthracis_str._A2012 Bacillus anthracis str. A2012]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3TYA OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3TYA FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=78H:7,8-DIHYDROPTEROATE'>78H</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.611Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1tws|1tws]]</td></tr>
| | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=78H:7,8-DIHYDROPTEROATE'>78H</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">folP, BAS0071, BA_0071, GBAA_0071 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=191218 Bacillus anthracis A2012])</td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3tya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tya OCA], [https://pdbe.org/3tya PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3tya RCSB], [https://www.ebi.ac.uk/pdbsum/3tya PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3tya ProSAT]</span></td></tr> |
| <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Dihydropteroate_synthase Dihydropteroate synthase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.5.1.15 2.5.1.15] </span></td></tr>
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| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3tya FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3tya OCA], [http://pdbe.org/3tya PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3tya RCSB], [http://www.ebi.ac.uk/pdbsum/3tya PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3tya ProSAT]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/Q81VW8_BACAN Q81VW8_BACAN] |
| The sulfonamide antibiotics inhibit dihydropteroate synthase (DHPS), a key enzyme in the folate pathway of bacteria and primitive eukaryotes. However, resistance mutations have severely compromised the usefulness of these drugs. We report structural, computational, and mutagenesis studies on the catalytic and resistance mechanisms of DHPS. By performing the enzyme-catalyzed reaction in crystalline DHPS, we have structurally characterized key intermediates along the reaction pathway. Results support an S(N)1 reaction mechanism via formation of a novel cationic pterin intermediate. We also show that two conserved loops generate a substructure during catalysis that creates a specific binding pocket for p-aminobenzoic acid, one of the two DHPS substrates. This substructure, together with the pterin-binding pocket, explains the roles of the conserved active-site residues and reveals how sulfonamide resistance arises.
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| Catalysis and sulfa drug resistance in dihydropteroate synthase.,Yun MK, Wu Y, Li Z, Zhao Y, Waddell MB, Ferreira AM, Lee RE, Bashford D, White SW Science. 2012 Mar 2;335(6072):1110-4. PMID:22383850<ref>PMID:22383850</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 3tya" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Dihydropteroate synthase|Dihydropteroate synthase]] | | *[[Dihydropteroate synthase 3D structures|Dihydropteroate synthase 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Bacillus anthracis a2012]] | | [[Category: Bacillus anthracis str. A2012]] |
| [[Category: Dihydropteroate synthase]] | | [[Category: Large Structures]] |
| [[Category: White, S W]] | | [[Category: White SW]] |
| [[Category: Yun, M K]] | | [[Category: Yun M-K]] |
| [[Category: Anthracis]]
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| [[Category: Dihydropteroate]]
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| [[Category: Folate biosynthesis]]
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| [[Category: Pterine]]
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| [[Category: Tim barrel]]
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| [[Category: Transferase]]
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