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[[Image:1emk.gif|left|200px]]<br />
<applet load="1emk" size="450" color="white" frame="true" align="right" spinBox="true"
caption="1emk, resolution 2.1&Aring;" />
'''GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT'''<br />


==About this Structure==
==GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT==
1EMK is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Structure known Active Site: CCY. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1EMK OCA].  
<StructureSection load='1emk' size='340' side='right'caption='[[1emk]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[1emk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1EMK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1EMK FirstGlance]. <br>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.1&#8491;</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CCY:2-(1-AMINO-2-MERCAPTO-ETHYL)-5-(4-HYDROXY-BENZYL)-3-(ETHANOYL)-3,5-DIHYDRO-IMIDAZOL-4-ONE'>CCY</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1emk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1emk OCA], [https://pdbe.org/1emk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1emk RCSB], [https://www.ebi.ac.uk/pdbsum/1emk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1emk ProSAT]</span></td></tr>
</table>
== Function ==
[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
== Evolutionary Conservation ==
[[Image:Consurf_key_small.gif|200px|right]]
Check<jmol>
  <jmolCheckbox>
    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/em/1emk_consurf.spt"</scriptWhenChecked>
    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview03.spt</scriptWhenUnchecked>
    <text>to colour the structure by Evolutionary Conservation</text>
  </jmolCheckbox>
</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1emk ConSurf].
<div style="clear:both"></div>


==Reference==
==See Also==
The structural basis for spectral variations in green fluorescent protein., Palm GJ, Zdanov A, Gaitanaris GA, Stauber R, Pavlakis GN, Wlodawer A, Nat Struct Biol. 1997 May;4(5):361-5. PMID:[http://ispc.weizmann.ac.il//pmbin/getpm?pmid=9145105 9145105]
*[[Green Fluorescent Protein 3D structures|Green Fluorescent Protein 3D structures]]
__TOC__
</StructureSection>
[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
[[Category: Single protein]]
[[Category: Large Structures]]
[[Category: Palm, G.]]
[[Category: Palm G]]
[[Category: Wlodawer, A.]]
[[Category: Wlodawer A]]
[[Category: Zdanov, A.]]
[[Category: Zdanov A]]
[[Category: beta-barrel]]
[[Category: bioluminescence]]
[[Category: fluorescent protein]]
[[Category: luminescence]]
 
''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Mon Nov  5 16:10:59 2007''

Latest revision as of 02:56, 21 November 2024

GREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANTGREEN FLUORESCENT PROTEIN FROM AEQUOREA VICTORIA, MUTANT

Structural highlights

1emk is a 1 chain structure with sequence from Aequorea victoria. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.1Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

GFP_AEQVI Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

1emk, resolution 2.10Å

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