5tum: Difference between revisions

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New page: '''Unreleased structure''' The entry 5tum is ON HOLD until Paper Publication Authors: Park, J., Tolia, N.H. Description: Crystal structure of tetracycline destructase Tet(56) [[Categor...
 
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'''Unreleased structure'''


The entry 5tum is ON HOLD  until Paper Publication
==Crystal structure of tetracycline destructase Tet(56)==
 
<StructureSection load='5tum' size='340' side='right'caption='[[5tum]], [[Resolution|resolution]] 3.30&Aring;' scene=''>
Authors: Park, J., Tolia, N.H.
== Structural highlights ==
 
<table><tr><td colspan='2'>[[5tum]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Legionella_longbeachae Legionella longbeachae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5TUM OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5TUM FirstGlance]. <br>
Description: Crystal structure of tetracycline destructase Tet(56)
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.299&#8491;</td></tr>
[[Category: Unreleased Structures]]
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene></td></tr>
[[Category: Park, J]]
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5tum FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5tum OCA], [https://pdbe.org/5tum PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5tum RCSB], [https://www.ebi.ac.uk/pdbsum/5tum PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5tum ProSAT]</span></td></tr>
[[Category: Tolia, N.H]]
</table>
== Function ==
[https://www.uniprot.org/uniprot/TET56_LEGLN TET56_LEGLN] An FAD-requiring monooxygenase active on tetracycline antibiotic and some of its derivatives, which leads to their inactivation (PubMed:26097034). Expression in E.coli confers high resistance to tetracycline and oxytetracycline, does not confer resistance to minocycline or tigecycline. The reaction requires NADPH (PubMed:26097034). Expression in L.pneumophila confers resistance to tetracycline (PubMed:28481346). Degrades and confers resistance to tetracycline and chlortetracycline (PubMed:26097034, PubMed:28481346).<ref>PMID:26097034</ref> <ref>PMID:28481346</ref>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Legionella longbeachae]]
[[Category: Park J]]
[[Category: Tolia NH]]

Latest revision as of 17:23, 6 March 2024

Crystal structure of tetracycline destructase Tet(56)Crystal structure of tetracycline destructase Tet(56)

Structural highlights

5tum is a 2 chain structure with sequence from Legionella longbeachae. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.299Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

TET56_LEGLN An FAD-requiring monooxygenase active on tetracycline antibiotic and some of its derivatives, which leads to their inactivation (PubMed:26097034). Expression in E.coli confers high resistance to tetracycline and oxytetracycline, does not confer resistance to minocycline or tigecycline. The reaction requires NADPH (PubMed:26097034). Expression in L.pneumophila confers resistance to tetracycline (PubMed:28481346). Degrades and confers resistance to tetracycline and chlortetracycline (PubMed:26097034, PubMed:28481346).[1] [2]

References

  1. ↑ Forsberg KJ, Patel S, Wencewicz TA, Dantas G. The Tetracycline Destructases: A Novel Family of Tetracycline-Inactivating Enzymes. Chem Biol. 2015 Jul 23;22(7):888-97. doi: 10.1016/j.chembiol.2015.05.017. Epub, 2015 Jun 18. PMID:26097034 doi:http://dx.doi.org/10.1016/j.chembiol.2015.05.017
  2. ↑ Park J, Gasparrini AJ, Reck MR, Symister CT, Elliott JL, Vogel JP, Wencewicz TA, Dantas G, Tolia NH. Plasticity, dynamics, and inhibition of emerging tetracycline resistance enzymes. Nat Chem Biol. 2017 May 8. doi: 10.1038/nchembio.2376. PMID:28481346 doi:http://dx.doi.org/10.1038/nchembio.2376

5tum, resolution 3.30Å

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