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==Crystal structure of alkaline invertase InvA from Anabaena sp. PCC 7120 complexed with glucose== | ==Crystal structure of alkaline invertase InvA from Anabaena sp. PCC 7120 complexed with glucose== | ||
<StructureSection load='5goq' size='340' side='right' caption='[[5goq]], [[Resolution|resolution]] 2.75Å' scene=''> | <StructureSection load='5goq' size='340' side='right'caption='[[5goq]], [[Resolution|resolution]] 2.75Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5goq]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GOQ OCA]. For a <b>guided tour on the structure components</b> use [ | <table><tr><td colspan='2'>[[5goq]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Nostoc_sp._PCC_7120_=_FACHB-418 Nostoc sp. PCC 7120 = FACHB-418]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GOQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GOQ FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.75Å</td></tr> | ||
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | |||
<tr id=' | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5goq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5goq OCA], [https://pdbe.org/5goq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5goq RCSB], [https://www.ebi.ac.uk/pdbsum/5goq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5goq ProSAT]</span></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q8YWS9_NOSS1 Q8YWS9_NOSS1] | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Invertases catalyze the hydrolysis of sucrose to glucose and fructose, thereby playing a key role in primary metabolism and plant development. According to the optimum pH, invertases are classified into acid invertases (Ac-Invs) and alkaline/neutral invertases (A/N-Invs), which share no sequence homology. Compared with Ac-Invs that have been extensively studied, the structure and catalytic mechanism of A/N-Invs remain unknown. Here we report the crystal structures of Anabaena alkaline invertase InvA, which was proposed to be the ancestor of modern plant A/N-Invs. These structures are the first in the GH100 family. InvA exists as a hexamer in both crystal and solution. Each subunit consists of an (alpha/alpha)6 barrel core structure in addition to an insertion of three helices. A couple of structures in complex with the substrate or products enabled us to assign the subsites -1 and +1 specifically binding glucose and fructose, respectively. Structural comparison combined with enzymatic assays indicated that Asp-188 and Glu-414 are putative catalytic residues. Further analysis of the substrate binding pocket demonstrated that InvA possesses a stringent substrate specificity toward the alpha1,2-glycosidic bond of sucrose. Together, we suggest that InvA and homologs represent a novel family of glucosidases. | |||
Structural Analysis of the Catalytic Mechanism and Substrate Specificity of Anabaena Alkaline Invertase InvA Reveals a Novel Glucosidase.,Xie J, Cai K, Hu HX, Jiang YL, Yang F, Hu PF, Cao DD, Li WF, Chen Y, Zhou CZ J Biol Chem. 2016 Dec 2;291(49):25667-25677. Epub 2016 Oct 24. PMID:27777307<ref>PMID:27777307</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
</div> | |||
<div class="pdbe-citations 5goq" style="background-color:#fffaf0;"></div> | |||
==See Also== | |||
*[[Invertase|Invertase]] | |||
== References == | |||
<references/> | |||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Cai | [[Category: Nostoc sp. PCC 7120 = FACHB-418]] | ||
[[Category: Chen | [[Category: Cai K]] | ||
[[Category: Hu | [[Category: Chen Y]] | ||
[[Category: Hu | [[Category: Hu HX]] | ||
[[Category: Jiang | [[Category: Hu PF]] | ||
[[Category: Xie | [[Category: Jiang YL]] | ||
[[Category: Yang | [[Category: Xie J]] | ||
[[Category: Zhou | [[Category: Yang F]] | ||
[[Category: Zhou CZ]] | |||