5gs3: Difference between revisions
New page: '''Unreleased structure''' The entry 5gs3 is ON HOLD Authors: Kim, J.H., Song, D.H., Youn, S.J., Kim, J.W., Cho, G., Lee, H., Lee, J.O. Description: Crystal structure of diabody [[Cate... |
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==Crystal structure of diabody== | |||
<StructureSection load='5gs3' size='340' side='right'caption='[[5gs3]], [[Resolution|resolution]] 1.70Å' scene=''> | |||
== Structural highlights == | |||
<table><tr><td colspan='2'>[[5gs3]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5GS3 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5GS3 FirstGlance]. <br> | |||
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.698Å</td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5gs3 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5gs3 OCA], [https://pdbe.org/5gs3 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5gs3 RCSB], [https://www.ebi.ac.uk/pdbsum/5gs3 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5gs3 ProSAT]</span></td></tr> | |||
</table> | |||
<div style="background-color:#fffaf0;"> | |||
== Publication Abstract from PubMed == | |||
Building a sophisticated protein nano-assembly requires a method for linking protein components in a predictable and stable structure. Diabodies are engineered antibody fragments that are composed of two Fv domains connected by short peptide linkers. They are attractive candidates for mediators in assembling protein nano-structures because they can simultaneously bind to two different proteins and are rigid enough to be crystallized. However, comparison of previous crystal structures demonstrates that there is substantial structural diversity in the Fv interface region of diabodies and, therefore, reliable prediction of its structure is not trivial. Here, we present the crystal structures of ten mono- and bi-specific diabodies. We found that changing an arginine residue in the Fv interface to threonine greatly reduced the structural diversity of diabodies. We also found that one of the bispecific diabodies underwent an unexpected process of chain swapping yielding a non-functional monospecific diabody. In order to further reduce structural flexibility and prevent chain shuffling, we introduced disulfide bridges in the Fv interface regions. The disulfide-bridged diabodies have rigid and predictable structures and may have applications in crystallizing proteins, analyzing cryo-electron microscopic images and building protein nano-assemblies. | |||
Crystal structures of mono- and bi-specific diabodies and reduction of their structural flexibility by introduction of disulfide bridges at the Fv interface.,Kim JH, Song DH, Youn SJ, Kim JW, Cho G, Kim SC, Lee H, Jin MS, Lee JO Sci Rep. 2016 Sep 29;6:34515. doi: 10.1038/srep34515. PMID:27682821<ref>PMID:27682821</ref> | |||
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | |||
[[Category: | </div> | ||
[[Category: | <div class="pdbe-citations 5gs3" style="background-color:#fffaf0;"></div> | ||
[[Category: Kim | == References == | ||
[[Category: | <references/> | ||
[[Category: | __TOC__ | ||
[[Category: Lee | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: Cho G]] | |||
[[Category: Kim JH]] | |||
[[Category: Kim JW]] | |||
[[Category: Lee H]] | |||
[[Category: Lee JO]] | |||
[[Category: Song DH]] | |||
[[Category: Youn SJ]] | |||