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==THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE==
==THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE==
<StructureSection load='379d' size='340' side='right' caption='[[379d]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
<StructureSection load='379d' size='340' side='right'caption='[[379d]], [[Resolution|resolution]] 3.10&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[379d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=379D OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=379D FirstGlance]. <br>
<table><tr><td colspan='2'>[[379d]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=379D OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=379D FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.1&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=379d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=379d OCA], [http://pdbe.org/379d PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=379d RCSB], [http://www.ebi.ac.uk/pdbsum/379d PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=379d ProSAT]</span></td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CO:COBALT+(II)+ION'>CO</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=379d FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=379d OCA], [https://pdbe.org/379d PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=379d RCSB], [https://www.ebi.ac.uk/pdbsum/379d PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=379d ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
We have captured an 8.7 A conformational change that takes place in the cleavage site of the hammerhead ribozyme during self-cleavage, using X-ray crystallography combined with physical and chemical trapping techniques. This rearrangement brings the hammerhead ribozyme from the ground state into a conformation that is poised to form the transition state geometry required for hammerhead RNA self-cleavage. Use of a 5'-C-methylated ribose adjacent to the cleavage site permits this ordinarily transient conformational change to be kinetically trapped and observed crystallographically after initiating the hammerhead ribozyme reaction in the crystal. Cleavage of the corresponding unmodified hammerhead ribozyme in the crystal under otherwise identical conditions is faster than in solution, indicating that we have indeed trapped a catalytically relevant intermediate form of this RNA enzyme.
The structural basis of hammerhead ribozyme self-cleavage.,Murray JB, Terwey DP, Maloney L, Karpeisky A, Usman N, Beigelman L, Scott WG Cell. 1998 Mar 6;92(5):665-73. PMID:9506521<ref>PMID:9506521</ref>
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 379d" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Ribozyme|Ribozyme]]
*[[Ribozyme|Ribozyme]]
== References ==
*[[Ribozyme 3D structures|Ribozyme 3D structures]]
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Beigelman, L]]
[[Category: Large Structures]]
[[Category: Karpeisky, A]]
[[Category: Beigelman L]]
[[Category: Maloney, L]]
[[Category: Karpeisky A]]
[[Category: Murray, J B]]
[[Category: Maloney L]]
[[Category: Scott, W G]]
[[Category: Murray JB]]
[[Category: Terwey, D P]]
[[Category: Scott WG]]
[[Category: Usman, N]]
[[Category: Terwey DP]]
[[Category: Catalytic rna]]
[[Category: Usman N]]
[[Category: Loop]]
[[Category: Ribozyme]]
[[Category: Rna hammerhead ribozyme]]

Latest revision as of 11:39, 7 February 2024

THE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGETHE STRUCTURAL BASIS OF HAMMERHEAD RIBOZYME SELF-CLEAVAGE

Structural highlights

379d is a 2 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 3.1Å
Ligands:
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

See Also

379d, resolution 3.10Å

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