4neo: Difference between revisions
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==Structure of BlmI, a type-II acyl-carrier-protein from Streptomyces verticillus involved in bleomycin biosynthesis== | ==Structure of BlmI, a type-II acyl-carrier-protein from Streptomyces verticillus involved in bleomycin biosynthesis== | ||
<StructureSection load='4neo' size='340' side='right' caption='[[4neo]], [[Resolution|resolution]] 2.10Å' scene=''> | <StructureSection load='4neo' size='340' side='right'caption='[[4neo]], [[Resolution|resolution]] 2.10Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4neo]] is a 1 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4neo]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_verticillus Streptomyces verticillus]. This structure supersedes the now removed PDB entry [http://oca.weizmann.ac.il/oca-bin/send-pdb?obs=1&id=4i4d 4i4d]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4NEO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4NEO FirstGlance]. <br> | ||
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MLY:N-DIMETHYL-LYSINE'>MLY</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PG0:2-(2-METHOXYETHOXY)ETHANOL'>PG0</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4neo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4neo OCA], [https://pdbe.org/4neo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4neo RCSB], [https://www.ebi.ac.uk/pdbsum/4neo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4neo ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | |||
[https://www.uniprot.org/uniprot/Q9XC48_9ACTN Q9XC48_9ACTN] | |||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Streptomyces verticillus | [[Category: Large Structures]] | ||
[[Category: Babnigg | [[Category: Streptomyces verticillus]] | ||
[[Category: Bearden | [[Category: Babnigg G]] | ||
[[Category: Bigelow | [[Category: Bearden J]] | ||
[[Category: Bingman | [[Category: Bigelow L]] | ||
[[Category: Bruno | [[Category: Bingman C]] | ||
[[Category: Cuff | [[Category: Bruno CJP]] | ||
[[Category: Joachimiak | [[Category: Cuff ME]] | ||
[[Category: Lohman | [[Category: Joachimiak A]] | ||
[[Category: Lohman J]] | |||
[[Category: Ma | [[Category: Ma M]] | ||
[[Category: Phillips Jr GN]] | |||
[[Category: Phillips | [[Category: Shen B]] | ||
[[Category: Shen | [[Category: Yennamalli R]] | ||
[[Category: Yennamalli | |||
Latest revision as of 11:25, 11 January 2023
Structure of BlmI, a type-II acyl-carrier-protein from Streptomyces verticillus involved in bleomycin biosynthesisStructure of BlmI, a type-II acyl-carrier-protein from Streptomyces verticillus involved in bleomycin biosynthesis
Structural highlights
FunctionPublication Abstract from PubMedCarrier proteins (CPs) play a critical role in the biosynthesis of various natural products, especially in nonribosomal peptide synthetase (NRPS) and polyketide synthase (PKS) enzymology, where the CPs are referred to as peptidyl-carrier proteins (PCPs) or acyl-carrier proteins (ACPs), respectively. CPs can either be a domain in large multifunctional polypeptides or standalone proteins, termed Type I and Type II, respectively. There have been many biochemical studies of the Type I PKS and NRPS CPs, and of Type II ACPs. However, recently a number of Type II PCPs have been found and biochemically characterized. In order to understand the possible interaction surfaces for combinatorial biosynthetic efforts we crystallized the first characterized and representative Type II PCP member, BlmI, from the bleomycin biosynthetic pathway from Streptomyces verticillus ATCC 15003. The structure is similar to CPs in general but most closely resembles PCPs. Comparisons with previously determined PCP structures in complex with catalytic domains reveals a common interaction surface. This surface is highly variable in charge and shape, which likely confers specificity for interactions. Previous nuclear magnetic resonance (NMR) analysis of a prototypical Type I PCP excised from the multimodular context revealed three conformational states. Comparison of the states with the structure of BlmI and other PCPs reveals that only one of the NMR states is found in other studies, suggesting the other two states may not be relevant. The state represented by the BlmI crystal structure can therefore serve as a model for both Type I and Type II PCPs. The crystal structure of BlmI as a model for nonribosomal peptide synthetase peptidyl carrier proteins.,Lohman JR, Ma M, Cuff ME, Bigelow L, Bearden J, Babnigg G, Joachimiak A, Phillips GN Jr, Shen B Proteins. 2014 Jul;82(7):1210-8. PMID:25050442[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References |
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