1nx0: Difference between revisions

{{Structure

|PDB= 1nx0 |SIZE=350|CAPTION= <scene name='initialview01'>1nx0</scene>, resolution 2.3&Aring;

|SITE=  

|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>

|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Hydrolase Hydrolase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.22.52 and 3.4.22.53 3.4.22.52 and 3.4.22.53] </span>

|GENE= CAPNS1 OR CAPN4 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9823 Sus scrofa])

|DOMAIN=

|RELATEDENTRY=[[1nx1|1NX1]], [[1nx2|1NX2]], [[1nx3|1NX3]]

|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1nx0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1nx0 OCA], [http://www.ebi.ac.uk/pdbsum/1nx0 PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1nx0 RCSB]</span>

 

 

==Overview==

The Ca(2+)-dependent cysteine protease calpain along with its endogenous inhibitor calpastatin is widely distributed. The interactions between calpain and calpastatin have been studied to better understand the nature of calpain inhibition by calpastatin, which can aid the design of small molecule inhibitors to calpain. Here we present the crystal structure of a complex between a calpastatin peptide and the calcium-binding domain VI of calpain. DIC19 is a 19 residue peptide, which corresponds to one of the three interacting domains of calpastatin, which is known to interact with domain VI of calpain. We present two crystal structures of DIC19 bound to domain VI of calpain, determined by molecular replacement methods to 2.5A and 2.2A resolution. In the process of crystallizing the inhibitor complex, a new native crystal form was identified which had the homodimer 2-fold axis along a crystallographic axis as opposed to the previously observed dimer in the asymmetric unit. The crystal structures of the native domain VI and its inhibitor PD150606 (3-(4-iodophenyl)-2-mercapto-(Z)-2-propenoic acid) complex were determined with the help of molecular replacement methods to 2.0A and 2.3A resolution, respectively. In addition, we built a homology model for the complex between domain IV and DIA19 peptide of calpastatin. Finally, we present a model for the calpastatin-inhibited calpain.

 

1NX0 is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1NX0 OCA].

 

A structural model for the inhibition of calpain by calpastatin: crystal structures of the native domain VI of calpain and its complexes with calpastatin peptide and a small molecule inhibitor., Todd B, Moore D, Deivanayagam CC, Lin GD, Chattopadhyay D, Maki M, Wang KK, Narayana SV, J Mol Biol. 2003 Apr 18;328(1):131-46. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12684003 12684003]

[[Category: Protein complex]]

[[Category: Chattopadhyay, D.]]

[[Category: Deivanayagam, C C.S.]]

[[Category: Lin, G D.]]

[[Category: Maki, M.]]

[[Category: Moore, D.]]

[[Category: Narayana, S V.L.]]

[[Category: Todd, B.]]

[[Category: Wang, K K.W.]]