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==Kinetic and structural characterization of the 4-oxalocrotonate tautomerase isozymes from Methylibium petroleiphilum==
==Kinetic and structural characterization of the 4-oxalocrotonate tautomerase isozymes from Methylibium petroleiphilum==
<StructureSection load='4fdx' size='340' side='right' caption='[[4fdx]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
<StructureSection load='4fdx' size='340' side='right'caption='[[4fdx]], [[Resolution|resolution]] 1.64&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4fdx]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Metpp Metpp]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FDX OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4FDX FirstGlance]. <br>
<table><tr><td colspan='2'>[[4fdx]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Methylibium_petroleiphilum_PM1 Methylibium petroleiphilum PM1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4FDX OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4FDX FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4faz|4faz]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.64&#8491;</td></tr>
<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Mpe_A3323 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=420662 METPP])</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4fdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fdx OCA], [https://pdbe.org/4fdx PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4fdx RCSB], [https://www.ebi.ac.uk/pdbsum/4fdx PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4fdx ProSAT]</span></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Oxaloacetate_tautomerase Oxaloacetate tautomerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=5.3.2.2 5.3.2.2] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4fdx FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4fdx OCA], [http://pdbe.org/4fdx PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4fdx RCSB], [http://www.ebi.ac.uk/pdbsum/4fdx PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4fdx ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/A2SL37_METPP A2SL37_METPP]
Methylibium petroleiphilum strain PM1 uses various petroleum products including the fuel additive methyl tert-butyl ether and straight chain and aromatic hydrocarbons as sole carbon and energy sources. It has two operons, dmpI and dmpII, that code for the enzymes in a pair of parallel meta-fission pathways. In order to understand the roles of the pathways, the 4-oxalocrotonate tautomerase (4-OT) isozyme from each pathway was characterized. Tautomerase I and tautomerase II have the lowest pairwise sequence identity (35%) among the isozyme pairs in the parallel pathways, and could offer insight into substrate preferences and pathway functions. The kinetic parameters of tautomerase I and tautomerase II were determined using 2-hydroxymuconate and 5-(methyl)-2-hydroxymuconate. Both tautomerase I and tautomerase II process the substrates, but with different efficiencies. Crystal structures were determined for both tautomerase I and tautomerase II, at 1.57 and 1.64A resolution, respectively. The backbones of tautomerase I and tautomerase II are highly similar, but have distinct active site environments. The results, in combination with those for other structurally and kinetically characterized 4-OT isozymes, suggest that tautomerase I catalyzes the tautomerization of both 2-hydroxymuconate and alkyl derivatives, whereas tautomerase II might specialize in other aromatic hydrocarbon metabolites.
 
Structural and kinetic characterization of two 4-oxalocrotonate tautomerases in Methylibium petroleiphilum strain PM1.,Terrell CR, Burks EA, Whitman CP, Hoffman DW Arch Biochem Biophys. 2013 Sep 1;537(1):113-24. doi: 10.1016/j.abb.2013.06.016., Epub 2013 Jul 4. PMID:23831510<ref>PMID:23831510</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4fdx" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Metpp]]
[[Category: Large Structures]]
[[Category: Oxaloacetate tautomerase]]
[[Category: Methylibium petroleiphilum PM1]]
[[Category: Hoffman, D W]]
[[Category: Hoffman DW]]
[[Category: Terrell, C R]]
[[Category: Terrell CR]]
[[Category: Whitman, C P]]
[[Category: Whitman CP]]
[[Category: Alpha/beta barrel]]
[[Category: Isomerase]]
[[Category: Tautomerase superfamily]]

Latest revision as of 14:15, 1 March 2024

Kinetic and structural characterization of the 4-oxalocrotonate tautomerase isozymes from Methylibium petroleiphilumKinetic and structural characterization of the 4-oxalocrotonate tautomerase isozymes from Methylibium petroleiphilum

Structural highlights

4fdx is a 2 chain structure with sequence from Methylibium petroleiphilum PM1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.64Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

A2SL37_METPP

4fdx, resolution 1.64Å

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