4f27: Difference between revisions
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==Crystal structures reveal the multi-ligand binding mechanism of the Staphylococcus aureus ClfB== | ==Crystal structures reveal the multi-ligand binding mechanism of the Staphylococcus aureus ClfB== | ||
<StructureSection load='4f27' size='340' side='right' caption='[[4f27]], [[Resolution|resolution]] 1.92Å' scene=''> | <StructureSection load='4f27' size='340' side='right'caption='[[4f27]], [[Resolution|resolution]] 1.92Å' scene=''> | ||
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[4f27]] is a 2 chain structure with sequence from [ | <table><tr><td colspan='2'>[[4f27]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Staphylococcus_aureus_subsp._aureus_MRSA252 Staphylococcus aureus subsp. aureus MRSA252]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4F27 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4F27 FirstGlance]. <br> | ||
</td></tr><tr id=' | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.917Å</td></tr> | ||
<tr id=' | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr> | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4f27 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4f27 OCA], [https://pdbe.org/4f27 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4f27 RCSB], [https://www.ebi.ac.uk/pdbsum/4f27 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4f27 ProSAT]</span></td></tr> | |||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[ | |||
</table> | </table> | ||
== Function == | == Function == | ||
[ | [https://www.uniprot.org/uniprot/CLFB_STAAR CLFB_STAAR] Cell surface-associated protein implicated in virulence by promoting bacterial attachment to both alpha- and beta-chains of human fibrinogen and inducing the formation of bacterial clumps. | ||
<div style="background-color:#fffaf0;"> | <div style="background-color:#fffaf0;"> | ||
== Publication Abstract from PubMed == | == Publication Abstract from PubMed == | ||
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__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: | [[Category: Homo sapiens]] | ||
[[Category: | [[Category: Large Structures]] | ||
[[Category: | [[Category: Staphylococcus aureus subsp. aureus MRSA252]] | ||
[[Category: | [[Category: Chen YG]] | ||
[[Category: | [[Category: Deng XM]] | ||
[[Category: | [[Category: Feng Y]] | ||
[[Category: | [[Category: Liu B]] | ||
[[Category: | [[Category: Liu L]] | ||
[[Category: | [[Category: Wang JW]] | ||
[[Category: | [[Category: Xiang H]] | ||
[[Category: | [[Category: Yang MJ]] | ||
Latest revision as of 11:20, 9 October 2024
Crystal structures reveal the multi-ligand binding mechanism of the Staphylococcus aureus ClfBCrystal structures reveal the multi-ligand binding mechanism of the Staphylococcus aureus ClfB
Structural highlights
FunctionCLFB_STAAR Cell surface-associated protein implicated in virulence by promoting bacterial attachment to both alpha- and beta-chains of human fibrinogen and inducing the formation of bacterial clumps. Publication Abstract from PubMedStaphylococcus aureus (S. aureus) pathogenesis is a complex process involving a diverse array of extracellular and cell wall components. ClfB, an MSCRAMM (Microbial Surface Components Recognizing Adhesive Matrix Molecules) family surface protein, described as a fibrinogen-binding clumping factor, is a key determinant of S. aureus nasal colonization, but the molecular basis for ClfB-ligand recognition remains unknown. In this study, we solved the crystal structures of apo-ClfB and its complexes with fibrinogen alpha (Fg alpha) and cytokeratin 10 (CK10) peptides. Structural comparison revealed a conserved glycine-serine-rich (GSR) ClfB binding motif (GSSGXGXXG) within the ligands, which was also found in other human proteins such as Engrailed protein, TCF20 and Dermokine proteins. Interaction between Dermokine and ClfB was confirmed by subsequent binding assays. The crystal structure of ClfB complexed with a 15-residue peptide derived from Dermokine revealed the same peptide binding mode of ClfB as identified in the crystal structures of ClfB-Fg alpha and ClfB-CK10. The results presented here highlight the multi-ligand binding property of ClfB, which is very distinct from other characterized MSCRAMMs to-date. The adherence of multiple peptides carrying the GSR motif into the same pocket in ClfB is reminiscent of MHC molecules. Our results provide a template for the identification of other molecules targeted by S. aureus during its colonization and infection. We propose that other MSCRAMMs like ClfA and SdrG also possess multi-ligand binding properties. Crystal Structures Reveal the Multi-Ligand Binding Mechanism of Staphylococcus aureus ClfB.,Xiang H, Feng Y, Wang J, Liu B, Chen Y, Liu L, Deng X, Yang M PLoS Pathog. 2012 Jun;8(6):e1002751. Epub 2012 Jun 14. PMID:22719251[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. See AlsoReferences
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