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==Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and glycerol==
==Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and glycerol==
<StructureSection load='4kt2' size='340' side='right' caption='[[4kt2]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
<StructureSection load='4kt2' size='340' side='right'caption='[[4kt2]], [[Resolution|resolution]] 1.80&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4kt2]] is a 8 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KT2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4KT2 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4kt2]] is a 8 chain structure with sequence from [https://en.wikipedia.org/wiki/Chromohalobacter_salexigens_DSM_3043 Chromohalobacter salexigens DSM 3043]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4KT2 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4KT2 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.798&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[4kpl|4kpl]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Mannonate_dehydratase Mannonate dehydratase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=4.2.1.8 4.2.1.8] </span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4kt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kt2 OCA], [https://pdbe.org/4kt2 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4kt2 RCSB], [https://www.ebi.ac.uk/pdbsum/4kt2 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4kt2 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4kt2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4kt2 OCA], [http://pdbe.org/4kt2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4kt2 RCSB], [http://www.ebi.ac.uk/pdbsum/4kt2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4kt2 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
[https://www.uniprot.org/uniprot/DMGD_CHRSD DMGD_CHRSD] Has low dehydratase activity with D-mannonate and D-gluconate, suggesting that these are not physiological substrates and that it has no significant role in the in vivo degradation of these compounds. Has no detectable activity with a panel of 70 other acid sugars (in vitro).<ref>PMID:24697546</ref>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Mannonate dehydratase]]
[[Category: Chromohalobacter salexigens DSM 3043]]
[[Category: Almo, S C]]
[[Category: Large Structures]]
[[Category: Fedorov, A A]]
[[Category: Almo SC]]
[[Category: Fedorov, E V]]
[[Category: Fedorov AA]]
[[Category: Gerlt, J A]]
[[Category: Fedorov EV]]
[[Category: Wichelecki, D]]
[[Category: Gerlt JA]]
[[Category: D-mannonate dehydratase]]
[[Category: Wichelecki D]]
[[Category: Enolase fold]]
[[Category: Hydrolase]]

Latest revision as of 19:05, 20 September 2023

Crystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and glycerolCrystal structure of d-mannonate dehydratase from chromohalobacter salexigens complexed with mg and glycerol

Structural highlights

4kt2 is a 8 chain structure with sequence from Chromohalobacter salexigens DSM 3043. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.798Å
Ligands:, , ,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

DMGD_CHRSD Has low dehydratase activity with D-mannonate and D-gluconate, suggesting that these are not physiological substrates and that it has no significant role in the in vivo degradation of these compounds. Has no detectable activity with a panel of 70 other acid sugars (in vitro).[1]

References

  1. ↑ Wichelecki DJ, Balthazor BM, Chau AC, Vetting MW, Fedorov AA, Fedorov EV, Lukk T, Patskovsky YV, Stead MB, Hillerich BS, Seidel RD, Almo SC, Gerlt JA. Discovery of function in the enolase superfamily: D-mannonate and d-gluconate dehydratases in the D-mannonate dehydratase subgroup. Biochemistry. 2014 Apr 29;53(16):2722-31. doi: 10.1021/bi500264p. Epub 2014 Apr, 15. PMID:24697546 doi:http://dx.doi.org/10.1021/bi500264p

4kt2, resolution 1.80Å

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