4rh4: Difference between revisions

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==Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing==
==Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing==
<StructureSection load='4rh4' size='340' side='right' caption='[[4rh4]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
<StructureSection load='4rh4' size='340' side='right'caption='[[4rh4]], [[Resolution|resolution]] 1.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4rh4]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RH4 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4RH4 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4rh4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Alcaligenes_faecalis Alcaligenes faecalis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4RH4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4RH4 FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.6&#8491;</td></tr>
<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1paz|1paz]], [[3nyk|3nyk]], [[1pza|1pza]], [[1pzc|1pzc]]</td></tr>
<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4rh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rh4 OCA], [http://pdbe.org/4rh4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4rh4 RCSB], [http://www.ebi.ac.uk/pdbsum/4rh4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4rh4 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4rh4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4rh4 OCA], [https://pdbe.org/4rh4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4rh4 RCSB], [https://www.ebi.ac.uk/pdbsum/4rh4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4rh4 ProSAT]</span></td></tr>
</table>
</table>
== Function ==
== Function ==
[[http://www.uniprot.org/uniprot/AZUP_ALCFA AZUP_ALCFA]] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. Serves as a direct electron donor to the nitrite reductase.  
[https://www.uniprot.org/uniprot/AZUP_ALCFA AZUP_ALCFA] This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. Serves as a direct electron donor to the nitrite reductase.
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
The copper(II) centre of the blue copper protein pseudoazurin from Alcaligenes faecalis has been substituted by zinc(II) via denaturing the protein, chelation and removal of copper and refolding the apoprotein, followed by the addition of an aqueous solution of ZnCl2. Vapour-diffusion experiments produced colourless hexagonal crystals (space group P65), which when cryocooled had unit-cell parameters a = b = 49.01, c = 98.08 A. Diffraction data collected at 100 K using a copper sealed tube were phased by the weak anomalous signal of five S atoms and one Zn atom. The structure was fitted manually and refined to 1.6 A resolution. The zinc-substituted protein exhibits similar overall geometry to the native structure with copper. Zn(2+) binds more strongly to its four ligand atoms (His40 N(delta1), Cys78 S(gamma), His81 N(delta1) and Met86 S(delta)) and retains the tetrahedral arrangement, although the structure is less distorted than the native copper protein.
 
Zinc-substituted pseudoazurin solved by S/Zn-SAD phasing.,Gessmann R, Papadovasilaki M, Drougkas E, Petratos K Acta Crystallogr F Struct Biol Commun. 2015 Jan 1;71(Pt 1):19-23. doi:, 10.1107/S2053230X14025552. Epub 2015 Jan 1. PMID:25615962<ref>PMID:25615962</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4rh4" style="background-color:#fffaf0;"></div>


==See Also==
==See Also==
*[[Pseudoazurin|Pseudoazurin]]
*[[Pseudoazurin|Pseudoazurin]]
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Gessmann, R]]
[[Category: Alcaligenes faecalis]]
[[Category: Petratos, K]]
[[Category: Large Structures]]
[[Category: Beta-sandwich]]
[[Category: Gessmann R]]
[[Category: Divalent metal-ion]]
[[Category: Petratos K]]
[[Category: Metal binding protein]]
[[Category: Metalloprotein]]
[[Category: Sad]]

Latest revision as of 15:52, 1 March 2024

Zinc-substituted pseudoazurin solved by S/Zn-SAD phasingZinc-substituted pseudoazurin solved by S/Zn-SAD phasing

Structural highlights

4rh4 is a 1 chain structure with sequence from Alcaligenes faecalis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 1.6Å
Ligands:,
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

AZUP_ALCFA This soluble electron transfer copper protein is required for the inactivation of copper-containing nitrite reductase in the presence of oxygen. Serves as a direct electron donor to the nitrite reductase.

See Also

4rh4, resolution 1.60Å

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