4mt5: Difference between revisions

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==Crystal structure of Mub-RV==
==Crystal structure of Mub-RV==
<StructureSection load='4mt5' size='340' side='right' caption='[[4mt5]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
<StructureSection load='4mt5' size='340' side='right'caption='[[4mt5]], [[Resolution|resolution]] 2.60&Aring;' scene=''>
== Structural highlights ==
== Structural highlights ==
<table><tr><td colspan='2'>[[4mt5]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MT5 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4MT5 FirstGlance]. <br>
<table><tr><td colspan='2'>[[4mt5]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Limosilactobacillus_reuteri_subsp._suis Limosilactobacillus reuteri subsp. suis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=4MT5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=4MT5 FirstGlance]. <br>
</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3i57|3i57]]</td></tr>
</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.6&#8491;</td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=4mt5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mt5 OCA], [http://pdbe.org/4mt5 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=4mt5 RCSB], [http://www.ebi.ac.uk/pdbsum/4mt5 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=4mt5 ProSAT]</span></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=4mt5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=4mt5 OCA], [https://pdbe.org/4mt5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=4mt5 RCSB], [https://www.ebi.ac.uk/pdbsum/4mt5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=4mt5 ProSAT]</span></td></tr>
</table>
</table>
<div style="background-color:#fffaf0;">
== Function ==
== Publication Abstract from PubMed ==
[https://www.uniprot.org/uniprot/F8KCE4_LIMR5 F8KCE4_LIMR5]
The mucus layer covering the gastrointestinal (GI) epithelium is critical in selecting and maintaining homeostatic interactions with our gut bacteria. However, the underpinning mechanisms of these interactions are not understood. Here, we provide structural and functional insights into the canonical mucus-binding protein (MUB), a multi-repeat cell-surface adhesin found in Lactobacillus inhabitants of the GI tract. X-ray crystallography together with small-angle X-ray scattering demonstrated a 'beads on a string' arrangement of repeats, generating 174 nm long protein fibrils, as shown by atomic force microscopy. Each repeat consists of tandemly arranged Ig- and mucin-binding protein (MucBP) modules. The binding of full-length MUB was confined to mucus via multiple interactions involving terminal sialylated mucin glycans. While individual MUB domains showed structural similarity to fimbrial proteins from Gram-positive pathogens, the particular organization of MUB provides a structural explanation for the mechanisms in which lactobacilli have adapted to their host niche by maximizing interactions with the mucus receptors, potentiating the retention of bacteria within the mucus layer. Together, this study reveals functional and structural features which may affect tropism of microbes across mucus and along the GI tract, providing unique insights into the mechanisms adopted by commensals and probiotics to adapt to the mucosal environment.
 
Structural basis for adaptation of lactobacilli to gastrointestinal mucus.,Etzold S, Kober OI, Mackenzie DA, Tailford LE, Gunning AP, Walshaw J, Hemmings AM, Juge N Environ Microbiol. 2014 Mar;16(3):888-903. doi: 10.1111/1462-2920.12377. Epub, 2014 Jan 23. PMID:24373178<ref>PMID:24373178</ref>
 
From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 4mt5" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
__TOC__
</StructureSection>
</StructureSection>
[[Category: Etzold, S]]
[[Category: Large Structures]]
[[Category: Hemmings, A M]]
[[Category: Limosilactobacillus reuteri subsp. suis]]
[[Category: Juge, N]]
[[Category: Etzold S]]
[[Category: Cell-surface]]
[[Category: Hemmings AM]]
[[Category: Mucin binding]]
[[Category: Juge N]]
[[Category: Protein binding]]
[[Category: Ubiquitin-like beta-grasp fold]]

Latest revision as of 15:29, 1 March 2024

Crystal structure of Mub-RVCrystal structure of Mub-RV

Structural highlights

4mt5 is a 2 chain structure with sequence from Limosilactobacillus reuteri subsp. suis. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Method:X-ray diffraction, Resolution 2.6Å
Resources:FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

F8KCE4_LIMR5

4mt5, resolution 2.60Å

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