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| ==Crystal structure of acetylcholine bound to a prokaryotic pentameric ligand-gated ion channel, ELIC== | | ==Crystal structure of acetylcholine bound to a prokaryotic pentameric ligand-gated ion channel, ELIC== |
| <StructureSection load='3rqw' size='340' side='right' caption='[[3rqw]], [[Resolution|resolution]] 2.91Å' scene=''> | | <StructureSection load='3rqw' size='340' side='right'caption='[[3rqw]], [[Resolution|resolution]] 2.91Å' scene=''> |
| == Structural highlights == | | == Structural highlights == |
| <table><tr><td colspan='2'>[[3rqw]] is a 10 chain structure with sequence from [http://en.wikipedia.org/wiki/Dicd3 Dicd3]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3RQW FirstGlance]. <br> | | <table><tr><td colspan='2'>[[3rqw]] is a 10 chain structure with sequence from [https://en.wikipedia.org/wiki/Dickeya_dadantii_3937 Dickeya dadantii 3937]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3RQW OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=3RQW FirstGlance]. <br> |
| </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ACH:ACETYLCHOLINE'>ACH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr> | | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.913Å</td></tr> |
| <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[3rqu|3rqu]]</td></tr> | | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACH:ACETYLCHOLINE'>ACH</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene></td></tr> |
| <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Dda3937_00520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=198628 DICD3])</td></tr> | | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=3rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqw OCA], [https://pdbe.org/3rqw PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=3rqw RCSB], [https://www.ebi.ac.uk/pdbsum/3rqw PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=3rqw ProSAT]</span></td></tr> |
| <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=3rqw FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=3rqw OCA], [http://pdbe.org/3rqw PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=3rqw RCSB], [http://www.ebi.ac.uk/pdbsum/3rqw PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=3rqw ProSAT]</span></td></tr> | |
| </table> | | </table> |
| <div style="background-color:#fffaf0;">
| | == Function == |
| == Publication Abstract from PubMed == | | [https://www.uniprot.org/uniprot/E0SJQ4_DICD3 E0SJQ4_DICD3] |
| ELIC, the pentameric ligand-gated ion channel from Erwinia chrysanthemi, is a prototype for Cys-loop receptors. Here we show that acetylcholine is a competitive antagonist for ELIC. We determine the acetylcholine-ELIC cocrystal structure to a 2.9-A resolution and find that acetylcholine binding to an aromatic cage at the subunit interface induces a significant contraction of loop C and other structural rearrangements in the extracellular domain. The side chain of the pore-lining residue F247 reorients and the pore size consequently enlarges, but the channel remains closed. We attribute the inability of acetylcholine to activate ELIC primarily to weak cation-pi and electrostatic interactions in the pocket, because an acetylcholine derivative with a simple quaternary-to-tertiary ammonium substitution activates the channel. This study presents a compelling case for understanding the structural underpinning of the functional relationship between agonism and competitive antagonism in the Cys-loop receptors, providing a new framework for developing novel therapeutic drugs.
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| Structure of the pentameric ligand-gated ion channel ELIC cocrystallized with its competitive antagonist acetylcholine.,Pan J, Chen Q, Willenbring D, Yoshida K, Tillman T, Kashlan OB, Cohen A, Kong XP, Xu Y, Tang P Nat Commun. 2012 Mar 6;3:714. doi: 10.1038/ncomms1703. PMID:22395605<ref>PMID:22395605</ref>
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| From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
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| </div>
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| <div class="pdbe-citations 3rqw" style="background-color:#fffaf0;"></div>
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| ==See Also== | | ==See Also== |
| *[[Ion channels|Ion channels]] | | *[[Ion channels 3D structures|Ion channels 3D structures]] |
| == References ==
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| <references/>
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| __TOC__ | | __TOC__ |
| </StructureSection> | | </StructureSection> |
| [[Category: Dicd3]] | | [[Category: Dickeya dadantii 3937]] |
| [[Category: Chen, Q]] | | [[Category: Large Structures]] |
| [[Category: Cohen, A]] | | [[Category: Chen Q]] |
| [[Category: Kong, X P]] | | [[Category: Cohen A]] |
| [[Category: Pan, J J]] | | [[Category: Kong XP]] |
| [[Category: Tang, P]] | | [[Category: Pan JJ]] |
| [[Category: Xu, Y]] | | [[Category: Tang P]] |
| [[Category: Yoshida, K]] | | [[Category: Xu Y]] |
| [[Category: Ion channel]]
| | [[Category: Yoshida K]] |
| [[Category: Membrane]]
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| [[Category: Transport protein]]
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