1mws: Difference between revisions

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-[[Image:1mws.jpg|left|200px]]
- {{Structure
+==Structure of nitrocefin acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2.00 A resolution.==
-|PDB= 1mws |SIZE=350|CAPTION= <scene name='initialview01'>1mws</scene>, resolution 2.00&Aring;
+<StructureSection load='1mws' size='340' side='right'caption='[[1mws]], [[Resolution|resolution]] 2.00&Aring;' scene=''>
-|SITE=
+== Structural highlights ==
-|LIGAND= <scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NC1:NITROCEFIN+ACYL-SERINE'>NC1</scene>
+<table><tr><td colspan='2'>[[1mws]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=1MWS FirstGlance]. <br>
-|ACTIVITY=
+</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2&#8491;</td></tr>
-|GENE=
+<tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=NC1:NITROCEFIN+ACYL-SERINE'>NC1</scene></td></tr>
-|DOMAIN=
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=1mws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mws OCA], [https://pdbe.org/1mws PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=1mws RCSB], [https://www.ebi.ac.uk/pdbsum/1mws PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=1mws ProSAT]</span></td></tr>
-|RELATEDENTRY=[[1mwr|1MWR]], [[1mwt|1MWT]], [[1mwu|1MWU]], [[1mwx|1MWX]]
+</table>
-|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1mws FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1mws OCA], [http://www.ebi.ac.uk/pdbsum/1mws PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=1mws RCSB]</span>
+== Function ==
-}}
+[https://www.uniprot.org/uniprot/Q93IC2_STAAU Q93IC2_STAAU]
+== Evolutionary Conservation ==
+[[Image:Consurf_key_small.gif|200px|right]]
+Check<jmol>
+  <jmolCheckbox>
+    <scriptWhenChecked>; select protein; define ~consurf_to_do selected; consurf_initial_scene = true; script "/wiki/ConSurf/mw/1mws_consurf.spt"</scriptWhenChecked>
+    <scriptWhenUnchecked>script /wiki/extensions/Proteopedia/spt/initialview01.spt</scriptWhenUnchecked>
+    <text>to colour the structure by Evolutionary Conservation</text>
+  </jmolCheckbox>
+</jmol>, as determined by [http://consurfdb.tau.ac.il/ ConSurfDB]. You may read the [[Conservation%2C_Evolutionary|explanation]] of the method and the full data available from [http://bental.tau.ac.il/new_ConSurfDB/main_output.php?pdb_ID=1mws ConSurf].
+<div style="clear:both"></div>
-'''Structure of nitrocefin acyl-Penicillin binding protein 2a from methicillin resistant Staphylococcus aureus strain 27r at 2.00 A resolution.'''
+==See Also==
+*[[Penicillin-binding protein 3D structures|Penicillin-binding protein 3D structures]]
+__TOC__
-==Overview==
+</StructureSection>
-The multiple antibiotic resistance of methicillin-resistant strains of Staphylococcus aureus (MRSA) has become a major clinical problem worldwide. The key determinant of the broad-spectrum beta-lactam resistance in MRSA strains is the penicillin-binding protein 2a (PBP2a). Because of its low affinity for beta-lactams, PBP2a provides transpeptidase activity to allow cell wall synthesis at beta-lactam concentrations that inhibit the beta-lactam-sensitive PBPs normally produced by S. aureus. The crystal structure of a soluble derivative of PBP2a has been determined to 1.8 A resolution and provides the highest resolution structure for a high molecular mass PBP. Additionally, structures of the acyl-PBP complexes of PBP2a with nitrocefin, penicillin G and methicillin allow, for the first time, a comparison of an apo and acylated resistant PBP. An analysis of the PBP2a active site in these forms reveals the structural basis of its resistance and identifies features in newly developed beta-lactams that are likely important for high affinity binding.
+[[Category: Large Structures]]
-==About this Structure==
-MWS is a [[Single protein]] structure of sequence from [http://en.wikipedia.org/wiki/Staphylococcus_aureus Staphylococcus aureus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1MWS OCA].
-==Reference==
-Structural basis for the beta lactam resistance of PBP2a from methicillin-resistant Staphylococcus aureus., Lim D, Strynadka NC, Nat Struct Biol. 2002 Nov;9(11):870-6. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/12389036 12389036]
-[[Category: Single protein]]
 [[Category: Staphylococcus aureus]]
-[[Category: Lim, D C.]]
+[[Category: Lim DC]]
-[[Category: Strynadka, N C.J.]]
+[[Category: Strynadka NCJ]]
-[[Category: beta-lactam]]
-[[Category: d,d-carboxypeptidase]]
-[[Category: d,d-transpeptidase]]
-[[Category: nitrocefin]]
-[[Category: penicillin binding protein]]
-''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun Mar 30 22:22:15 2008''